3TMJ

Joint X-ray/neutron structure of human carbonic anhydrase II at pH 7.8

3TMJ の概要

• エントリーDOI: 10.2210/pdb3tmj/pdb
• 分子名称: Carbonic anhydrase 2, ZINC ION (3 entities in total)
• 機能のキーワード: h/d exchanged, joint neutron/x-ray refinement, lyase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29136.19

構造登録者

Fisher, Z. (登録日: 2011-08-31, 公開日: 2011-11-09, 最終更新日: 2023-09-13)

主引用文献

Fisher, Z.,Kovalevsky, A.Y.,Mustyakimov, M.,Silverman, D.N.,McKenna, R.,Langan, P.
Neutron Structure of Human Carbonic Anhydrase II: A Hydrogen-Bonded Water Network "Switch" Is Observed between pH 7.8 and 10.0.
Biochemistry, 50:9421-9423, 2011

PubMed Abstract: The neutron structure of wild-type human carbonic anhydrase II at pH 7.8 has been determined to 2.0 Å resolution. Detailed analysis and comparison to the previously determined structure at pH 10.0 show important differences in the protonation of key catalytic residues in the active site as well as a rearrangement of the H-bonded water network. For the first time, a completed H-bonded network stretching from the Zn-bound solvent to the proton shuttling residue, His64, has been directly observed.

PubMed: 21988105
DOI: 10.1021/bi201487b

実験手法

NEUTRON DIFFRACTION (2 Å)
X-RAY DIFFRACTION