3TLM

Crystal Structure of Endoplasmic Reticulum Ca2+-ATPase (SERCA) From Bovine Muscle

3TLM の概要

• エントリーDOI: 10.2210/pdb3tlm/pdb
• 関連するPDBエントリー: 1SU4 1T5S
• 分子名称: Sarcoplasmic/endoplasmic reticulum calcium ATPase 1, CALCIUM ION, MAGNESIUM ION, ... (6 entities in total)
• 機能のキーワード: serca, ca-atpase, calcium transporter, ca, fast-twitch muscle, hydrolase
• 由来する生物種: Bos taurus (bovine,cow,domestic cattle,domestic cow)
• 細胞内の位置: Endoplasmic reticulum membrane; Multi-pass membrane protein: Q0VCY0
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 109995.06

構造登録者

Sacchetto, R.,Bertipaglia, I.,Giannetti, S.,Cendron, L.,Mascarello, F.,Damiani, E.,Carafoli, E.,Zanotti, G. (登録日: 2011-08-30, 公開日: 2012-03-21, 最終更新日: 2023-09-13)

主引用文献

Sacchetto, R.,Bertipaglia, I.,Giannetti, S.,Cendron, L.,Mascarello, F.,Damiani, E.,Carafoli, E.,Zanotti, G.
Crystal structure of sarcoplasmic reticulum Ca(2+)-ATPase (SERCA) from bovine muscle.
J.Struct.Biol., 178:38-44, 2012

PubMed Abstract: The SERCA pump, a membrane protein of about 110kDa, transports two Ca(2+) ions per ATP hydrolyzed from the cytoplasm to the lumen of the sarcoplasmic reticulum. In muscle cells, its ability to remove Ca(2+) from the cytosol induces relaxation. The transport mechanism employed by the enzyme from rabbit muscle has been extensively studied, and several crystal structures representing different conformational states are available. However, no structure of the pump from other sources is known. In this paper we describe the crystal structure of the bovine enzyme, crystallized in the E1 conformation and determined at 2.9Å resolution. The overall molecular model is very similar to that of the rabbit enzyme, as expected by the high amino acid sequence identity. Nevertheless, the bovine enzyme has reduced catalytic activity with respect to the rabbit enzyme. Subtle structural modifications, in particular in the region of the long loop that protrudes into the SR lumen connecting transmembrane α-helices M7 and M8, may explain the difference.

PubMed: 22387132
DOI: 10.1016/j.jsb.2012.02.008

実験手法

X-RAY DIFFRACTION (2.95 Å)