3THV

Crystal Structure of Bacillus DNA Polymerase I Large Fragment Bound to DNA and ddATP-dT in Closed Conformation

3THV の概要

• エントリーDOI: 10.2210/pdb3thv/pdb
• 関連するPDBエントリー: 3TAN 3TAP 3TAQ 3TAR 3TI0
• 分子名称: DNA polymerase I, 5'-D(*CP*CP*TP*GP*AP*CP*TP*CP*(2DA))-3', 5'-D(*C*AP*TP*TP*TP*GP*AP*GP*TP*CP*AP*GP*G)-3', ... (7 entities in total)
• 機能のキーワード: dna polymerase i, protein-dna complex, transferase-dna complex, transferase/dna
• 由来する生物種: Geobacillus
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 149600.53

構造登録者

Wang, W.,Beese, L.S. (登録日: 2011-08-19, 公開日: 2011-10-19, 最終更新日: 2023-09-13)

主引用文献

Wang, W.,Hellinga, H.W.,Beese, L.S.
Structural evidence for the rare tautomer hypothesis of spontaneous mutagenesis.
Proc.Natl.Acad.Sci.USA, 108:17644-17648, 2011

PubMed Abstract: Even though high-fidelity polymerases copy DNA with remarkable accuracy, some base-pair mismatches are incorporated at low frequency, leading to spontaneous mutagenesis. Using high-resolution X-ray crystallographic analysis of a DNA polymerase that catalyzes replication in crystals, we observe that a C • A mismatch can mimic the shape of cognate base pairs at the site of incorporation. This shape mimicry enables the mismatch to evade the error detection mechanisms of the polymerase, which would normally either prevent mismatch incorporation or promote its nucleolytic excision. Movement of a single proton on one of the mismatched bases alters the hydrogen-bonding pattern such that a base pair forms with an overall shape that is virtually indistinguishable from a canonical, Watson-Crick base pair in double-stranded DNA. These observations provide structural evidence for the rare tautomer hypothesis of spontaneous mutagenesis, a long-standing concept that has been difficult to demonstrate directly.

PubMed: 22006298
DOI: 10.1073/pnas.1114496108

実験手法

X-RAY DIFFRACTION (1.611 Å)