3T72

PhoB(E)-Sigma70(4)-(RNAP-Betha-flap-tip-helix)-DNA Transcription Activation Sub-Complex

3T72 の概要

• エントリーDOI: 10.2210/pdb3t72/pdb
• 分子名称: Phosphate regulon transcriptional regulatory protein phoB, PHO BOX DNA (STRAND 1), PHO BOX DNA (STRAND 2), ... (4 entities in total)
• 機能のキーワード: winged-helix motif, transcription activation, dna-binding, transcription-dna complex, transcription/dna
• 由来する生物種: Escherichia coli; 詳細
• 細胞内の位置: Cytoplasm: P0AFJ5
• タンパク質・核酸の鎖数: 50
• 化学式量合計: 501148.49

構造登録者

Blanco, A.G.,Canals, A.,Bernues, J.,Sola, M.,Coll, M. (登録日: 2011-07-29, 公開日: 2011-09-21, 最終更新日: 2024-05-22)

主引用文献

Blanco, A.G.,Canals, A.,Bernues, J.,Sola, M.,Coll, M.
The structure of a transcription activation subcomplex reveals how sigma (70) is recruited to PhoB promoters.
Embo J., 30:3776-3785, 2011

PubMed Abstract: PhoB is a two-component response regulator that activates transcription by interacting with the σ(70) subunit of the E. coli RNA polymerase in promoters in which the -35 σ(70)-recognition element is replaced by the pho box. The crystal structure of a transcription initiation subcomplex that includes the σ(4) domain of σ(70) fused with the RNA polymerase β subunit flap tip helix, the PhoB effector domain and the pho box DNA reveals how σ(4) recognizes the upstream pho box repeat. As with the -35 element, σ(4) achieves this recognition through the N-terminal portion of its DNA recognition helix, but contact with the DNA major groove is less extensive. Unexpectedly, the same recognition helix contacts the transactivation loop and helices α2 and α3 of PhoB. This result shows a simple and elegant mechanism for polymerase recruitment to pho box promoters in which the lost -35 element contacts are compensated by new ones with the activator. In addition, σ(4) is reoriented, thereby suggesting a remodelling mechanism for transcription initiation.

PubMed: 21829166
DOI: 10.1038/emboj.2011.271

実験手法

X-RAY DIFFRACTION (4.33 Å)