3SZT

Quorum Sensing Control Repressor, QscR, Bound to N-3-oxo-dodecanoyl-L-Homoserine Lactone

3SZT の概要

• エントリーDOI: 10.2210/pdb3szt/pdb
• 分子名称: Quorum-sensing control repressor, N-3-OXO-DODECANOYL-L-HOMOSERINE LACTONE, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: quorum sensing acyl-homoserine lactone, helix-turn-helix, transcription factor, 3-oxo-c12 hsl, transcription
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 55188.07

構造登録者

Churchill, M.E.A.,Lintz, M.J. (登録日: 2011-07-19, 公開日: 2011-09-28, 最終更新日: 2024-04-03)

主引用文献

Lintz, M.J.,Oinuma, K.,Wysoczynski, C.L.,Greenberg, E.P.,Churchill, M.E.
Crystal structure of QscR, a Pseudomonas aeruginosa quorum sensing signal receptor.
Proc.Natl.Acad.Sci.USA, 108:15763-15768, 2011

PubMed Abstract: Acyl-homoserine lactone (AHL) quorum sensing controls gene expression in hundreds of Proteobacteria including a number of plant and animal pathogens. Generally, the AHL receptors are members of a family of related transcription factors, and although they have been targets for development of antivirulence therapeutics there is very little structural information about this class of bacterial receptors. We have determined the structure of the transcription factor, QscR, bound to N-3-oxo-dodecanoyl-homoserine lactone from the opportunistic human pathogen Pseudomonas aeruginosa at a resolution of 2.55 Å. The ligand-bound QscR is a dimer with a unique symmetric "cross-subunit" arrangement containing multiple dimerization interfaces involving both domains of each subunit. The QscR dimer appears poised to bind DNA. Predictions about signal binding and dimerization contacts were supported by studies of mutant QscR proteins in vivo. The acyl chain of the AHL is in close proximity to the dimerization interfaces. Our data are consistent with an allosteric mechanism of signal transmission in the regulation of DNA binding and thus virulence gene expression.

PubMed: 21911405
DOI: 10.1073/pnas.1112398108

実験手法

X-RAY DIFFRACTION (2.55 Å)