3SYJ

Crystal structure of the Haemophilus influenzae Hap adhesin

3SYJ の概要

• エントリーDOI: 10.2210/pdb3syj/pdb
• 分子名称: Adhesion and penetration protein autotransporter (2 entities in total)
• 機能のキーワード: bacterial aggregation and biofilm formation, self-associating autotransporter (saat), oligomerization, beta helix fold, membrane, cell adhesion
• 由来する生物種: Haemophilus influenzae
• 細胞内の位置: Adhesion and penetration protein autotransporter: Periplasm . Adhesion and penetration protein: Secreted. Adhesion and penetration protein translocator: Cell outer membrane ; Multi-pass membrane protein : P45387
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 112138.53

構造登録者

Meng, G. (登録日: 2011-07-18, 公開日: 2011-08-24, 最終更新日: 2024-10-16)

主引用文献

Meng, G.,Spahich, N.,Kenjale, R.,Waksman, G.,St Geme III, J.W.
Crystal structure of the Haemophilus influenzae Hap adhesin reveals an intercellular oligomerization mechanism for bacterial aggregation
Embo J., 30:3864-3874, 2011

PubMed Abstract: Bacterial biofilms are complex microbial communities that are common in nature and are being recognized increasingly as an important determinant of bacterial virulence. However, the structural determinants of bacterial aggregation and eventual biofilm formation have been poorly defined. In Gram-negative bacteria, a major subgroup of extracellular proteins called self-associating autotransporters (SAATs) can mediate cell-cell adhesion and facilitate biofilm formation. In this study, we used the Haemophilus influenzae Hap autotransporter as a prototype SAAT to understand how bacteria associate with each other. The crystal structure of the H. influenzae Hap(S) passenger domain (harbouring the SAAT domain) was determined to 2.2 Å by X-ray crystallography, revealing an unprecedented intercellular oligomerization mechanism for cell-cell interaction. The C-terminal SAAT domain folds into a triangular-prism-like structure that can mediate Hap-Hap dimerization and higher degrees of multimerization through its F1-F2 edge and F2 face. The intercellular multimerization can give rise to massive buried surfaces that are required for overcoming the repulsive force between cells, leading to bacterial cell-cell interaction and formation of complex microcolonies.

PubMed: 21841773
DOI: 10.1038/emboj.2011.279

実験手法

X-RAY DIFFRACTION (2.2 Å)