3SXU

Structure of the E. coli SSB-DNA polymerase III interface

3SXU の概要

• エントリーDOI: 10.2210/pdb3sxu/pdb
• 分子名称: DNA polymerase III subunit chi, DNA polymerase III subunit psi, SSB peptide, ... (4 entities in total)
• 機能のキーワード: dna replication, chi binds to ssb and psi, transferase
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 32858.10

構造登録者

Marceau, A.H.,Keck, J.L. (登録日: 2011-07-15, 公開日: 2011-09-21, 最終更新日: 2023-09-13)

主引用文献

Marceau, A.H.,Bahng, S.,Massoni, S.C.,George, N.P.,Sandler, S.J.,Marians, K.J.,Keck, J.L.
Structure of the SSB-DNA polymerase III interface and its role in DNA replication.
Embo J., 30:4236-4247, 2011

PubMed Abstract: Interactions between single-stranded DNA-binding proteins (SSBs) and the DNA replication machinery are found in all organisms, but the roles of these contacts remain poorly defined. In Escherichia coli, SSB's association with the χ subunit of the DNA polymerase III holoenzyme has been proposed to confer stability to the replisome and to aid delivery of primers to the lagging-strand DNA polymerase. Here, the SSB-binding site on χ is identified crystallographically and biochemical and cellular studies delineate the consequences of destabilizing the χ/SSB interface. An essential role for the χ/SSB interaction in lagging-strand primer utilization is not supported. However, sequence changes in χ that block complex formation with SSB lead to salt-dependent uncoupling of leading- and lagging-strand DNA synthesis and to a surprising obstruction of the leading-strand DNA polymerase in vitro, pointing to roles for the χ/SSB complex in replisome establishment and maintenance. Destabilization of the χ/SSB complex in vivo produces cells with temperature-dependent cell cycle defects that appear to arise from replisome instability.

PubMed: 21857649
DOI: 10.1038/emboj.2011.305

実験手法

X-RAY DIFFRACTION (1.85 Å)