3SQW

Structure of Mss116p (NTE deletion) bound to ssRNA and AMP-PNP

3SQW の概要

• エントリーDOI: 10.2210/pdb3sqw/pdb
• 関連するPDBエントリー: 3I5X 3I5Y 3I61 3I62 3SQX
• 分子名称: ATP-dependent RNA helicase MSS116, mitochondrial, RNA (5'-R(P*UP*UP*UP*UP*UP*UP*UP*UP*UP*U)-3'), PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, ... (5 entities in total)
• 機能のキーワード: reca fold, rna dependent atpase, rna helicase, mitochondrion, hydrolase-rna complex, hydrolase/rna
• 由来する生物種: Saccharomyces cerevisiae S288c (Baker's yeast); 詳細
• 細胞内の位置: Mitochondrion matrix: P15424
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 69781.51

構造登録者

Del Campo, M.,Lambowitz, A.M. (登録日: 2011-07-06, 公開日: 2011-10-12, 最終更新日: 2023-09-13)

主引用文献

Mohr, G.,Del Campo, M.,Turner, K.G.,Gilman, B.,Wolf, R.Z.,Lambowitz, A.M.
High-Throughput Genetic Identification of Functionally Important Regions of the Yeast DEAD-Box Protein Mss116p.
J.Mol.Biol., 413:952-972, 2011

PubMed Abstract: The Saccharomyces cerevisiae DEAD-box protein Mss116p is a general RNA chaperone that functions in splicing mitochondrial group I and group II introns. Recent X-ray crystal structures of Mss116p in complex with ATP analogs and single-stranded RNA show that the helicase core induces a bend in the bound RNA, as in other DEAD-box proteins, while a C-terminal extension (CTE) induces a second bend, resulting in RNA crimping. Here, we illuminate these structures by using high-throughput genetic selections, unigenic evolution, and analyses of in vivo splicing activity to comprehensively identify functionally important regions and permissible amino acid substitutions throughout Mss116p. The functionally important regions include those containing conserved sequence motifs involved in ATP and RNA binding or interdomain interactions, as well as previously unidentified regions, including surface loops that may function in protein-protein interactions. The genetic selections recapitulate major features of the conserved helicase motifs seen in other DEAD-box proteins but also show surprising variations, including multiple novel variants of motif III (SAT). Patterns of amino acid substitutions indicate that the RNA bend induced by the helicase core depends on ionic and hydrogen-bonding interactions with the bound RNA; identify a subset of critically interacting residues; and indicate that the bend induced by the CTE results primarily from a steric block. Finally, we identified two conserved regions-one the previously noted post II region in the helicase core and the other in the CTE-that may help displace or sequester the opposite RNA strand during RNA unwinding.

PubMed: 21945532
DOI: 10.1016/j.jmb.2011.09.015

実験手法

X-RAY DIFFRACTION (1.909 Å)