3SQI

DNA binding domain of Ndc10

3SQI の概要

• エントリーDOI: 10.2210/pdb3sqi/pdb
• 分子名称: KLLA0E03807p, DNA (5'-D(P*TP*TP*AP*AP*TP*TP*TP*AP*TP*AP*AP*AP*AP*TP*T)-3'), DNA (5'-D(P*AP*AP*AP*TP*TP*TP*TP*AP*TP*AP*AP*AP*TP*TP*A)-3') (3 entities in total)
• 機能のキーワード: dna recombinase, dna binding, dna, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Kluyveromyces lactis (yeast); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 70681.95

構造登録者

Cho, U.S.,Harrison, S.C. (登録日: 2011-07-05, 公開日: 2011-12-07, 最終更新日: 2024-02-28)

主引用文献

Cho, U.S.,Harrison, S.C.
Ndc10 is a platform for inner kinetochore assembly in budding yeast.
Nat.Struct.Mol.Biol., 19:48-55, 2011

PubMed Abstract: Kinetochores link centromeric DNA to spindle microtubules and ensure faithful chromosome segregation during mitosis. In point-centromere yeasts, the CBF3 complex Skp1-Ctf13-(Cep3)(2)-(Ndc10)(2) recognizes a conserved centromeric DNA element through contacts made by Cep3 and Ndc10. We describe here the five-domain organization of Kluyveromyces lactis Ndc10 and the structure at 2.8 Å resolution of domains I-II (residues 1-402) bound to DNA. The structure resembles tyrosine DNA recombinases, although it lacks both endonuclease and ligase activities. Structural and biochemical data demonstrate that each subunit of the Ndc10 dimer binds a separate fragment of DNA, suggesting that Ndc10 stabilizes a DNA loop at the centromere. We describe in vitro association experiments showing that specific domains of Ndc10 interact with each of the known inner-kinetochore proteins or protein complexes in budding yeast. We propose that Ndc10 provides a central platform for inner-kinetochore assembly.

PubMed: 22139014
DOI: 10.1038/nsmb.2178

実験手法

X-RAY DIFFRACTION (2.8245 Å)