3SOH

Architecture of the Flagellar Rotor

3SOH の概要

• エントリーDOI: 10.2210/pdb3soh/pdb
• 関連するPDBエントリー: 1LKV 2HP7
• 分子名称: Flagellar motor switch protein FliM, Flagellar motor switch protein FliG (3 entities in total)
• 機能のキーワード: protein-protein complex, alpha/beta, motor protein
• 由来する生物種: Thermotoga maritima; 詳細
• 細胞内の位置: Cell inner membrane ; Peripheral membrane protein : Q9WZE6; Cell inner membrane ; Peripheral membrane protein ; Cytoplasmic side : Q9WY63
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 61496.69

構造登録者

Koushik, P.,Gonzalez-Bonet, G.,Bilwes, A.M.,Crane, B.R.,Blair, D. (登録日: 2011-06-30, 公開日: 2011-07-27, 最終更新日: 2023-09-13)

主引用文献

Paul, K.,Gonzalez-Bonet, G.,Bilwes, A.M.,Crane, B.R.,Blair, D.
Architecture of the flagellar rotor.
Embo J., 30:2962-2971, 2011

PubMed Abstract: Rotation and switching of the bacterial flagellum depends on a large rotor-mounted protein assembly composed of the proteins FliG, FliM and FliN, with FliG most directly involved in rotation. The crystal structure of a complex between the central domains of FliG and FliM, in conjunction with several biochemical and molecular-genetic experiments, reveals the arrangement of the FliG and FliM proteins in the rotor. A stoichiometric mismatch between FliG (26 subunits) and FliM (34 subunits) is explained in terms of two distinct positions for FliM: one where it binds the FliG central domain and another where it binds the FliG C-terminal domain. This architecture provides a structural framework for addressing the mechanisms of motor rotation and direction switching and for unifying the large body of data on motor performance. Recently proposed alternative models of rotor assembly, based on a subunit contact observed in crystals, are not supported by experiment.

PubMed: 21673656
DOI: 10.1038/emboj.2011.188

実験手法

X-RAY DIFFRACTION (3.5 Å)