3SN2

Crystal structure analysis of iron regulatory protein 1 in complex with transferrin receptor IRE B RNA

3SN2 の概要

• エントリーDOI: 10.2210/pdb3sn2/pdb
• 関連するPDBエントリー: 2IPY 3SNP
• 分子名称: Cytoplasmic aconitate hydratase, transferrin receptor iron regulatory element B RNA (3 entities in total)
• 機能のキーワード: rna binding, iron sulfur cluster binding, phosphorylation, lyase-rna complex, lyase/rna
• 由来する生物種: Oryctolagus cuniculus (rabbit)
• 細胞内の位置: Cytoplasm: Q01059
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 110022.26

構造登録者

Volz, K.,Selezneva, A.I.,Walden, W.E. (登録日: 2011-06-28, 公開日: 2011-11-23, 最終更新日: 2023-09-13)

主引用文献

Walden, W.E.,Selezneva, A.,Volz, K.
Accommodating variety in iron-responsive elements: Crystal structure of transferrin receptor 1 B IRE bound to iron regulatory protein 1.
Febs Lett., 586:32-35, 2012

PubMed Abstract: Iron responsive elements (IREs) are short stem-loop structures found in several mRNAs encoding proteins involved in cellular iron metabolism. Iron regulatory proteins (IRPs) control iron homeostasis through differential binding to the IREs, accommodating any sequence or structural variations that the IREs may present. Here we report the structure of IRP1 in complex with transferrin receptor 1 B (TfR B) IRE, and compare it to the complex with ferritin H (Ftn H) IRE. The two IREs are bound to IRP1 through nearly identical protein-RNA contacts, although their stem conformations are significantly different. These results support the view that binding of different IREs with IRP1 depends both on protein and RNA conformational plasticity, adapting to RNA variation while retaining conserved protein-RNA contacts.

PubMed: 22119729
DOI: 10.1016/j.febslet.2011.11.018

実験手法

X-RAY DIFFRACTION (2.99 Å)