3SH3

Crystal structure of a pro-inflammatory lectin from the seeds of Dioclea wilsonii STANDL

3SH3 の概要

• エントリーDOI: 10.2210/pdb3sh3/pdb
• 関連するPDBエントリー: 3RRD 3RS6
• 分子名称: Lectin alpha chain, MANGANESE (II) ION, CALCIUM ION, ... (7 entities in total)
• 機能のキーワード: legume lectin, pro-inflammatory effect, jelly-roll, carbohydrate binding protein
• 由来する生物種: Dioclea wilsonii (Wilson's clusterpea)
• 細胞内の位置: Vacuole, aleurone grain: P86624
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 26299.58

構造登録者

Rangel, T.B.A.,Rocha, B.A.M.,Bezerra, G.A.,Bezerra, M.J.B.,Nascimento, K.S.,Nagano, C.S.,Sampaio, A.H.,Assreuy, A.M.S.,Gruber, K.,Delatorre, P.,Cavada, B.S. (登録日: 2011-06-15, 公開日: 2011-10-05, 最終更新日: 2024-02-28)

主引用文献

Rangel, T.B.,Rocha, B.A.,Bezerra, G.A.,Assreuy, A.M.,Pires, A.F.,Nascimento, A.S.,Bezerra, M.J.,Nascimento, K.S.,Nagano, C.S.,Sampaio, A.H.,Gruber, K.,Delatorre, P.,Fernandes, P.M.,Cavada, B.S.
Crystal structure of a pro-inflammatory lectin from the seeds of Dioclea wilsonii Standl.
Biochimie, 94:525-532, 2012

PubMed Abstract: The crystal structure and pro-inflammatory property of a lectin from the seeds of Dioclea wilsonii (DwL) were analyzed to gain a better understanding of structure/function relationships of Diocleinae lectins. Following crystallization and structural determination by standard molecular replacement techniques, DwL was found to be a tetramer based on PISA analysis, and composed by two metal-binding sites per monomer and loops which are involved in molecular oligomerization. DwL presents 96% and 99% identity with two other previously described lectins of Dioclea rostrata (DRL) and Dioclea grandiflora (DGL). DwL differs structurally from DVL and DRL with regard to the conformation of the carbohydrate recognition domain and related biological activities. The structural analysis of DwL in comparison to other Diocleinae lectins can be related to the differences in the dose-dependent pro-inflammatory effect elicited in Wistar rats, probably via specific interactions with mast cells complex carbohydrate, resulting in significant paw edema. DwL appears to be involved in positive modulation of mast cell degranulation via recognition of surface carbohydrates. Since this recognition is dependent on site volume and CRD configuration, edematogenesis mediated by resident cells varies in potency and efficacy among different Diocleinae lectins.

PubMed: 21924319
DOI: 10.1016/j.biochi.2011.09.001

実験手法

X-RAY DIFFRACTION (2.3 Å)