3SFE

crystal structure of porcine mitochondrial respiratory complex II bound with oxaloacetate and thiabendazole

3SFE の概要

• エントリーDOI: 10.2210/pdb3sfe/pdb
• 関連するPDBエントリー: 1ZOY 1ZP0 3SFD
• 分子名称: Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial, PROTOPORPHYRIN IX CONTAINING FE, 2-(1,3-THIAZOL-4-YL)-1H-BENZIMIDAZOLE, ... (12 entities in total)
• 機能のキーワード: succinate:ubiquinone oxidoreductase, thiabendazole, oxidoreductase
• 由来する生物種: Sus scrofa (pigs); 詳細
• 細胞内の位置: Mitochondrion inner membrane; Peripheral membrane protein; Matrix side: Q0QF01; Mitochondrion inner membrane; Peripheral membrane protein; Matrix side (By similarity): Q007T0; Mitochondrion inner membrane; Multi-pass membrane protein: D0VWV4; Mitochondrion inner membrane; Multi-pass membrane protein (By similarity): A5GZW8
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 125903.88

構造登録者

Zhou, Q.J.,Zhai, Y.J.,Liu, M.,Sun, F. (登録日: 2011-06-13, 公開日: 2011-09-07, 最終更新日: 2024-10-30)

主引用文献

Zhou, Q.,Zhai, Y.,Lou, J.,Liu, M.,Pang, X.,Sun, F.
Thiabendazole inhibits ubiquinone reduction activity of mitochondrial respiratory complex II via a water molecule mediated binding feature.
Protein Cell, 2:531-542, 2011

PubMed Abstract: The mitochondrial respiratory complex II or succinate: ubiquinone oxidoreductase (SQR) is a key membrane complex in both the tricarboxylic acid cycle and aerobic respiration. Five disinfectant compounds were investigated with their potent inhibition effects on the ubiquinone reduction activity of the porcine mitochondrial SQR by enzymatic assay and crystallography. Crystal structure of the SQR bound with thiabendazole (TBZ) reveals a different inhibitor-binding feature at the ubiquinone binding site where a water molecule plays an important role. The obvious inhibitory effect of TBZ based on the biochemical data (IC(50) ~100 μmol/L) and the significant structure-based binding affinity calculation (~94 μmol/L) draw the suspicion of using TBZ as a good disinfectant compound for nematode infections treatment and fruit storage.

PubMed: 21822798
DOI: 10.1007/s13238-011-1079-1

実験手法

X-RAY DIFFRACTION (2.81 Å)