3SFD

crystal structure of porcine mitochondrial respiratory complex II bound with oxaloacetate and pentachlorophenol

Summary for 3SFD

• Entry DOI: 10.2210/pdb3sfd/pdb
• Related: 1ZOY 1ZP0
• Descriptor: Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial, PROTOPORPHYRIN IX CONTAINING FE, PENTACHLOROPHENOL, ... (12 entities in total)
• Functional Keywords: succinate, ubiquinone oxidoreductase, oxidoreductase
• Biological source: Sus scrofa (pigs); More
• Cellular location: Mitochondrion inner membrane; Peripheral membrane protein; Matrix side: Q0QF01; Mitochondrion inner membrane; Peripheral membrane protein; Matrix side (By similarity): Q007T0; Mitochondrion inner membrane; Multi-pass membrane protein: D0VWV4; Mitochondrion inner membrane; Multi-pass membrane protein (By similarity): A5GZW8
• Total number of polymer chains: 4
• Total formula weight: 125968.97

Authors

Zhou, Q.J.,Zhai, Y.J.,Liu, M.,Sun, F. (deposition date: 2011-06-13, release date: 2011-09-07, Last modification date: 2024-11-20)

Primary citation

Zhou, Q.,Zhai, Y.,Lou, J.,Liu, M.,Pang, X.,Sun, F.
Thiabendazole inhibits ubiquinone reduction activity of mitochondrial respiratory complex II via a water molecule mediated binding feature.
Protein Cell, 2:531-542, 2011

PubMed Abstract: The mitochondrial respiratory complex II or succinate: ubiquinone oxidoreductase (SQR) is a key membrane complex in both the tricarboxylic acid cycle and aerobic respiration. Five disinfectant compounds were investigated with their potent inhibition effects on the ubiquinone reduction activity of the porcine mitochondrial SQR by enzymatic assay and crystallography. Crystal structure of the SQR bound with thiabendazole (TBZ) reveals a different inhibitor-binding feature at the ubiquinone binding site where a water molecule plays an important role. The obvious inhibitory effect of TBZ based on the biochemical data (IC(50) ~100 μmol/L) and the significant structure-based binding affinity calculation (~94 μmol/L) draw the suspicion of using TBZ as a good disinfectant compound for nematode infections treatment and fruit storage.

PubMed: 21822798
DOI: 10.1007/s13238-011-1079-1

Experimental method

X-RAY DIFFRACTION (2.61 Å)