3S9K

Crystal structure of the Itk SH2 domain.

3S9K の概要

• エントリーDOI: 10.2210/pdb3s9k/pdb
• 関連するPDBエントリー: 1LUK 1LUN 2ETZ 2K79
• 分子名称: Tyrosine-protein kinase ITK/TSK, CITRIC ACID (3 entities in total)
• 機能のキーワード: proline isomerization, cis proline, domain swapped dimer, sh2 domain, transferase
• 由来する生物種: Mus musculus (mouse)
• 細胞内の位置: Cytoplasm: Q03526
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13732.40

構造登録者

Joseph, R.E.,Ginder, N.D.,Hoy, J.A.,Nix, J.C.,Fulton, B.D.,Honzatko, R.B.,Andreotti, A.H. (登録日: 2011-06-01, 公開日: 2012-02-08, 最終更新日: 2024-02-28)

主引用文献

Joseph, R.E.,Ginder, N.D.,Hoy, J.A.,Nix, J.C.,Fulton, D.B.,Honzatko, R.B.,Andreotti, A.H.
Structure of the interleukin-2 tyrosine kinase Src homology 2 domain; comparison between X-ray and NMR-derived structures.
Acta Crystallogr.,Sect.F, 68:145-153, 2012

PubMed Abstract: The crystal structure of the interleukin-2 tyrosine kinase Src homology domain (Itk SH2) is described and it is found that unlike in studies of this domain using NMR spectroscopy, cis-trans-prolyl isomerization is not readily detected in the crystal structure. Based on similarities between the Itk SH2 crystal form and the cis form of the Itk SH2 NMR structure, it is concluded that it is likely that the prolyl imide bond at least in part adopts the cis conformation in the crystal form. However, the lack of high-resolution data and the dynamic nature of the proline-containing loop mean that the precise imide-bond conformation cannot be determined and prolyl cis-trans isomerization in the crystal cannot be ruled out. Given the preponderance of structures that have been solved by X-ray crystallography in the Protein Data Bank, this result supports the notion that prolyl isomerization in folded proteins has been underestimated among known structures. Interestingly, while the precise status of the proline residue is ambiguous, Itk SH2 crystallizes as a domain-swapped dimer. The domain-swapped structure of Itk SH2 is similar to the domain-swapped SH2 domains of Grb2 and Nck, with domain swapping occurring at the β-meander region of all three SH2 domains. Thus, for Itk SH2 structural analysis by NMR spectroscopy and X-ray crystallography revealed very different structural features: proline isomerization versus domain-swapped dimerization, respectively.

PubMed: 22297986
DOI: 10.1107/S1744309111049761

実験手法

X-RAY DIFFRACTION (2.354 Å)