3S6T

Crystal Structure of insect beta-N-acetyl-D-hexosaminidase OfHex1 V327G complexed with PUGNAc

Summary for 3S6T

• Entry DOI: 10.2210/pdb3s6t/pdb
• Descriptor: N-acetylglucosaminidase, O-(2-ACETAMIDO-2-DEOXY D-GLUCOPYRANOSYLIDENE) AMINO-N-PHENYLCARBAMATE (3 entities in total)
• Functional Keywords: beta-n-acetyl-d-hexosaminidase, hydrolase
• Biological source: Ostrinia furnacalis (Asian corn borer)
• Total number of polymer chains: 1
• Total formula weight: 66104.44

Authors

Zhang, H.,Liu, T.,Yang, Q.,Shen, X. (deposition date: 2011-05-26, release date: 2012-04-11, Last modification date: 2024-11-20)

Primary citation

Liu, T.,Zhang, H.,Liu, F.,Chen, L.,Shen, X.,Yang, Q.
Active-pocket size differentiating insectile from bacterial chitinolytic beta-N-acetyl-D-hexosaminidases.
Biochem.J., 438:467-474, 2011

PubMed Abstract: Chitinolytic β-N-acetyl-D-hexosaminidase is a branch of the GH20 (glycoside hydrolase family 20) β-N-acetyl-D-hexosaminidases that is only distributed in insects and micro-organisms, and is therefore a potential target for the action of insecticides. PUGNAc [O-(2-acetamido-2-deoxy-D-glucopyransylidene)-amino-N-phenylcarbamate] was initially identified as an inhibitor against GH20 β-N-acetyl-D-hexosaminidases. So far no crystal structure of PUGNAc in complex with any GH20 β-N-acetyl-D-hexosaminidase has been reported. We show in the present study that the sensitivities of chitinolytic β-N-acetyl-D-hexosaminidases towards PUGNAc can vary by 100-fold, with the order being OfHex1 (Ostrinia furnacalis β-N-acetyl-D-hexosaminidase)PubMed: 21692744
DOI: 10.1042/BJ20110390

Experimental method

X-RAY DIFFRACTION (2.3 Å)