3S53

HIV-1 protease triple mutants V32I, I47V, V82I with antiviral drug darunavir in space group P212121

3S53 の概要

• エントリーDOI: 10.2210/pdb3s53/pdb
• 関連するPDBエントリー: 2HS1 2IDW 2IEN 3EBZ 3S43 3S54
• 分子名称: Protease, (3R,3AS,6AR)-HEXAHYDROFURO[2,3-B]FURAN-3-YL(1S,2R)-3-[[(4-AMINOPHENYL)SULFONYL](ISOBUTYL)AMINO]-1-BENZYL-2-HYDROXYPROPYLCARBAMATE, PHOSPHATE ION, ... (5 entities in total)
• 機能のキーワード: darunavir, hiv/aids, drug resistance, aspartic protease, molecular recognition, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
• 由来する生物種: Human immunodeficiency virus 1
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 23968.75

構造登録者

Tie, Y.-F.,Wang, Y.-F.,Weber, I.T. (登録日: 2011-05-20, 公開日: 2012-03-21, 最終更新日: 2023-09-13)

主引用文献

Tie, Y.,Wang, Y.F.,Boross, P.I.,Chiu, T.Y.,Ghosh, A.K.,Tozser, J.,Louis, J.M.,Harrison, R.W.,Weber, I.T.
Critical differences in HIV-1 and HIV-2 protease specificity for clinical inhibitors.
Protein Sci., 21:339-350, 2012

PubMed Abstract: Clinical inhibitor amprenavir (APV) is less effective on HIV-2 protease (PR₂) than on HIV-1 protease (PR₁). We solved the crystal structure of PR₂ with APV at 1.5 Å resolution to identify structural changes associated with the lowered inhibition. Furthermore, we analyzed the PR₁ mutant (PR(1M) ) with substitutions V32I, I47V, and V82I that mimic the inhibitor binding site of PR₂. PR(1M) more closely resembled PR₂ than PR₁ in catalytic efficiency on four substrate peptides and inhibition by APV, whereas few differences were seen for two other substrates and inhibition by saquinavir (SQV) and darunavir (DRV). High resolution crystal structures of PR(1M) with APV, DRV, and SQV were compared with available PR₁ and PR₂ complexes. Val/Ile32 and Ile/Val47 showed compensating interactions with SQV in PR(1M) and PR₁, however, Ile82 interacted with a second SQV bound in an extension of the active site cavity of PR(1M). Residues 32 and 82 maintained similar interactions with DRV and APV in all the enzymes, whereas Val47 and Ile47 had opposing effects in the two subunits. Significantly diminished interactions were seen for the aniline of APV bound in PR₁ (M) and PR₂ relative to the strong hydrogen bonds observed in PR₁, consistent with 15- and 19-fold weaker inhibition, respectively. Overall, PR(1M) partially replicates the specificity of PR₂ and gives insight into drug resistant mutations at residues 32, 47, and 82. Moreover, this analysis provides a structural explanation for the weaker antiviral effects of APV on HIV-2.

PubMed: 22238126
DOI: 10.1002/pro.2019

実験手法

X-RAY DIFFRACTION (1.5 Å)