3S4Z
Structure of a Y DNA-FANCI complex
Summary for 3S4Z
| Entry DOI | 10.2210/pdb3s4z/pdb |
| Descriptor | dna repair 1 (1 entity in total) |
| Functional Keywords | dna repair, dna binding protein |
| Biological source | Mus musculus (mouse) |
| Cellular location | Nucleus (By similarity): Q8K368 |
| Total number of polymer chains | 3 |
| Total formula weight | 441361.69 |
| Authors | Pavletich, N.P. (deposition date: 2011-05-20, release date: 2011-07-27, Last modification date: 2024-02-28) |
| Primary citation | Joo, W.,Xu, G.,Persky, N.S.,Smogorzewska, A.,Rudge, D.G.,Buzovetsky, O.,Elledge, S.J.,Pavletich, N.P. Structure of the FANCI-FANCD2 complex: insights into the Fanconi anemia DNA repair pathway. Science, 333:312-316, 2011 Cited by PubMed Abstract: Fanconi anemia is a cancer predisposition syndrome caused by defects in the repair of DNA interstrand cross-links (ICLs). Central to this pathway is the Fanconi anemia I-Fanconi anemia D2 (FANCI-FANCD2) (ID) complex, which is activated by DNA damage-induced phosphorylation and monoubiquitination. The 3.4 angstrom crystal structure of the ~300 kilodalton ID complex reveals that monoubiquitination and regulatory phosphorylation sites map to the I-D interface, suggesting that they occur on monomeric proteins or an opened-up complex and that they may serve to stabilize I-D heterodimerization. The 7.8 angstrom electron-density map of FANCI-DNA crystals and in vitro data show that each protein has binding sites for both single- and double-stranded DNA, suggesting that the ID complex recognizes DNA structures that result from the encounter of replication forks with an ICL. PubMed: 21764741DOI: 10.1126/science.1205805 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (7.8 Å) |
Structure validation
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