3S4G

Low Resolution Structure of STNV complexed with RNA

3S4G の概要

• エントリーDOI: 10.2210/pdb3s4g/pdb
• 関連するPDBエントリー: 1VTZ 3RQV
• 分子名称: Capsid protein, RNA (5'-R(P*AP*AP*A)-3'), RNA (5'-R(P*UP*UP*UP*U)-3') (3 entities in total)
• 機能のキーワード: protein-rna complex, low resolution structure, jelly-roll -sandwich, virus capsid protein, virus
• 由来する生物種: Satellite tobacco necrosis virus 1; 詳細
• 細胞内の位置: Virion : P03606
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 23862.01

構造登録者

Lane, S.W.,Dennis, C.A.,Lane, C.L.,Trinh, C.H.,Rizkallah, P.J.,Stockley, P.G.,Phillips, S.E.V. (登録日: 2011-05-19, 公開日: 2011-08-03, 最終更新日: 2024-02-28)

主引用文献

Lane, S.W.,Dennis, C.A.,Lane, C.L.,Trinh, C.H.,Rizkallah, P.J.,Stockley, P.G.,Phillips, S.E.
Construction and crystal structure of recombinant STNV capsids.
J.Mol.Biol., 413:41-50, 2011

PubMed Abstract: A codon-optimised gene has been expressed in Escherichia coli to produce the coat protein (CP) of the Satellite Tobacco Necrosis Virus. This protein assembles in vivo into capsids closely resembling those of the T=1 wild-type virus. These virus-like particles (VLPs) package the recombinant mRNA transcript and can be disassembled and reassembled using different buffer conditions. The X-ray crystal structure of the VLP has been solved and refined at 1.4 Å resolution and shown to be very similar to that of wild-type Satellite Tobacco Necrosis Virus, except that icosahedral symmetry constraints could be removed to reveal differences between subunits, presumably owing to crystal packing. An additional low-resolution X-ray crystal structure determination revealed well-ordered RNA fragments lodged near the inside surface of the capsid, close to basic clusters formed by the N-terminal helices that project into the interior of the particle. The RNA consists of multiple copies of a 3-bp helical stem, with a single unpaired base at the 3' end, and probably consists of a number of short stem-loops where the loop region is disordered. The arrangement of the RNA is different from that observed in other satellite viruses.

PubMed: 21839089
DOI: 10.1016/j.jmb.2011.07.062

実験手法

X-RAY DIFFRACTION (6 Å)