3RZD

RNA Polymerase II Initiation Complex with a 5-nt RNA

3RZD の概要

• エントリーDOI: 10.2210/pdb3rzd/pdb
• 関連するPDBエントリー: 3RZO 3S14 3S15 3S16 3S17 3S1M 3S1N 3S1Q 3S1R 3S2D 3S2H
• 分子名称: DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerases I, II, and III subunit RPABC4, RNA (5'-R(*AP*GP*AP*GP*G)-3'), ... (14 entities in total)
• 機能のキーワード: rna polymerase ii, initiation complex, transcription-rna-dna complex, transcription/rna/dna
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• 細胞内の位置: Nucleus: P04050 P08518 P16370 P20434 P20436 P38902; Nucleus, nucleolus : P40422 P27999 P22139; Cytoplasm : P20435
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 480686.35

構造登録者

Liu, X.,Bushnell, D.A.,Silva, D.A.,Huang, X.,Kornberg, R.D. (登録日: 2011-05-11, 公開日: 2011-08-10, 最終更新日: 2024-02-28)

主引用文献

Liu, X.,Bushnell, D.A.,Silva, D.A.,Huang, X.,Kornberg, R.D.
Initiation complex structure and promoter proofreading.
Science, 333:633-637, 2011

PubMed Abstract: The initiation of transcription by RNA polymerase II is a multistage process. X-ray crystal structures of transcription complexes containing short RNAs reveal three structural states: one with 2- and 3-nucleotide RNAs, in which only the 3'-end of the RNA is detectable; a second state with 4- and 5-nucleotide RNAs, with an RNA-DNA hybrid in a grossly distorted conformation; and a third state with RNAs of 6 nucleotides and longer, essentially the same as a stable elongating complex. The transition from the first to the second state correlates with a markedly reduced frequency of abortive initiation. The transition from the second to the third state correlates with partial "bubble collapse" and promoter escape. Polymerase structure is permissive for abortive initiation, thereby setting a lower limit on polymerase-promoter complex lifetime and allowing the dissociation of nonspecific complexes. Abortive initiation may be viewed as promoter proofreading, and the structural transitions as checkpoints for promoter control.

PubMed: 21798951
DOI: 10.1126/science.1206629

実験手法

X-RAY DIFFRACTION (3.3 Å)