3RO2

Structures of the LGN/NuMA complex

Summary for 3RO2

• Entry DOI: 10.2210/pdb3ro2/pdb
• Related: 3RO3
• Descriptor: G-protein-signaling modulator 2, peptide of Nuclear mitotic apparatus protein 1, GLYCEROL, ... (4 entities in total)
• Functional Keywords: tpr repeat, protein-protein interaction, protein-binding, protein binding
• Biological source: Mus musculus (mouse); More
• Cellular location: Cytoplasm (By similarity): Q8VDU0; Nucleus: Q14980
• Total number of polymer chains: 2
• Total formula weight: 41066.67

Authors

Shang, Y.,Wei, Z. (deposition date: 2011-04-25, release date: 2012-03-07, Last modification date: 2024-03-20)

Primary citation

Zhu, J.,Wen, W.,Zheng, Z.,Shang, Y.,Wei, Z.,Xiao, Z.,Pan, Z.,Du, Q.,Wang, W.,Zhang, M.
LGN/mInsc and LGN/NuMA complex structures suggest distinct functions in asymmetric cell division for the Par3/mInsc/LGN and G[alpha]i/LGN/NuMA pathways
Mol.Cell, 43:418-431, 2011

PubMed Abstract: Asymmetric cell division requires the establishment of cortical cell polarity and the orientation of the mitotic spindle along the axis of cell polarity. Evidence from invertebrates demonstrates that the Par3/Par6/aPKC and NuMA/LGN/Gαi complexes, which are thought to be physically linked by the adaptor protein mInscuteable (mInsc), play indispensable roles in this process. However, the molecular basis for the binding of LGN to NuMA and mInsc is poorly understood. The high-resolution structures of the LGN/NuMA and LGN/mInsc complexes presented here provide mechanistic insights into the distinct and highly specific interactions of the LGN TPRs with mInsc and NuMA. Structural comparisons, together with biochemical and cell biology studies, demonstrate that the interactions of NuMA and mInsc with LGN are mutually exclusive, with mInsc binding preferentially. Our results suggest that the Par3/mInsc/LGN and NuMA/LGN/Gαi complexes play sequential and partially overlapping roles in asymmetric cell division.

PubMed: 21816348
DOI: 10.1016/j.molcel.2011.07.011

Experimental method

X-RAY DIFFRACTION (2.3 Å)