3RNY

Crystal structure of human RSK1 C-terminal kinase domain

3RNY の概要

• エントリーDOI: 10.2210/pdb3rny/pdb
• 分子名称: Ribosomal protein S6 kinase alpha-1, SODIUM ION (3 entities in total)
• 機能のキーワード: protein kinase, autoinhibition, transferase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus: Q15418
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 77690.69

構造登録者

Li, D.,Fu, T.-M.,Nan, J.,Su, X.-D. (登録日: 2011-04-24, 公開日: 2012-04-25, 最終更新日: 2023-09-13)

主引用文献

Li, D.,Fu, T.M.,Nan, J.,Liu, C.,Li, L.F.,Su, X.D.
Structural basis for the autoinhibition of the C-terminal kinase domain of human RSK1.
Acta Crystallogr.,Sect.D, 68:680-685, 2012

PubMed Abstract: p90 ribosomal S6 kinases (RSKs) respond to various mitogen stimuli and comprise two distinct protein kinase domains. The C-terminal kinase domain (CTKD) receives signal from ERK1/2 and adopts an autoinhibitory mechanism. Here, the crystal structure of human RSK1 CTKD is reported at 2.7 Å resolution. The structure shows a standard kinase fold, with the catalytic residues in the ATP-binding cleft orientated in optimal conformations for phosphotransfer. The inactivation of the CTKD is conferred by an extra α-helix (αL), which occupies the substrate-binding groove. In combination with previous knowledge, this structure indicates that activation of RSK1 involves the removal of αL from the substrate-binding groove induced by ERK1/2 phosphorylation.

PubMed: 22683790
DOI: 10.1107/S0907444912007457

実験手法

X-RAY DIFFRACTION (2.7 Å)