3RMH

Crystal Structure of yeast telomere protein Cdc13 OB4

3RMH の概要

• エントリーDOI: 10.2210/pdb3rmh/pdb
• 分子名称: Yeast Cdc13 OB4 (2 entities in total)
• 機能のキーワード: ob fold, dimer, dimeric complex, protein binding
• 由来する生物種: Candida glabrata (Yeast)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 35088.29

構造登録者

Sun, J.,Yang, Y.,Lei, M. (登録日: 2011-04-20, 公開日: 2011-11-09, 最終更新日: 2024-02-28)

主引用文献

Yu, E.Y.,Sun, J.,Lei, M.,Lue, N.F.
Analyses of Candida Cdc13 Orthologues Revealed a Novel OB Fold Dimer Arrangement, Dimerization-Assisted DNA Binding, and Substantial Structural Differences between Cdc13 and RPA70.
Mol.Cell.Biol., 32:186-198, 2012

PubMed Abstract: The budding yeast Cdc13-Stn1-Ten1 complex is crucial for telomere protection and has been proposed to resemble the RPA complex structurally and functionally. The Cdc13 homologues in Candida species are unusually small and lack two conserved domains previously implicated in telomere regulation, thus raising interesting questions concerning the mechanisms and evolution of these proteins. In this report, we show that the unusually small Cdc13 homologue in Candida albicans is indeed a regulator of telomere lengths and that it associates with telomere DNA in vivo. We demonstrated high-affinity telomere DNA binding by C. tropicalis Cdc13 (CtCdc13) and found that dimerization of this protein through its OB4 domain is important for high-affinity DNA binding. Interestingly, CtCdc13-DNA complex formation appears to involve primarily recognition of multiple copies of a six-nucleotide element (GGATGT) that is shared by many Candida telomere repeats. We also determined the crystal structure of the OB4 domain of C. glabrata Cdc13, which revealed a novel mechanism of OB fold dimerization. The structure also exhibits marked differences to the C-terminal OB fold of RPA70, thus arguing against a close evolutionary kinship between these two proteins. Our findings provide new insights on the mechanisms and evolution of a critical telomere end binding protein.

PubMed: 22025677
DOI: 10.1128/MCB.05875-11

実験手法

X-RAY DIFFRACTION (1.902 Å)