3RK2

Truncated SNARE complex

3RK2 の概要

• エントリーDOI: 10.2210/pdb3rk2/pdb
• 関連するPDBエントリー: 3RK3
• 分子名称: Vesicle-associated membrane protein 2, Syntaxin-1A, Synaptosomal-associated protein 25, ... (6 entities in total)
• 機能のキーワード: snare proteins, membrane fusion, membrane protein-exocytosis complex, membrane protein/exocytosis
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein: P63027; Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein (By similarity): P32851; Cytoplasm, perinuclear region (By similarity): P60880 P60880
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 57628.70

構造登録者

Kuemmel, D.,Reinisch, K.M. (登録日: 2011-04-17, 公開日: 2011-07-27, 最終更新日: 2023-09-13)

主引用文献

Kummel, D.,Krishnakumar, S.S.,Radoff, D.T.,Li, F.,Giraudo, C.G.,Pincet, F.,Rothman, J.E.,Reinisch, K.M.
Complexin cross-links prefusion SNAREs into a zigzag array.
Nat.Struct.Mol.Biol., 18:927-933, 2011

PubMed Abstract: Complexin prevents SNAREs from releasing neurotransmitters until an action potential arrives at the synapse. To understand the mechanism for this inhibition, we determined the structure of complexin bound to a mimetic of a prefusion SNAREpin lacking the portion of the v-SNARE that zippers last to trigger fusion. The 'central helix' of complexin is anchored to one SNARE complex, while its 'accessory helix' extends away at ~45° and bridges to a second complex, occupying the vacant v-SNARE binding site to inhibit fusion. We expected the accessory helix to compete with the v-SNARE for t-SNARE binding but found instead that the interaction occurs intermolecularly. Thus, complexin organizes the SNAREs into a zigzag topology that, when interposed between the vesicle and plasma membranes, is incompatible with fusion.

PubMed: 21785414
DOI: 10.1038/nsmb.2101

実験手法

X-RAY DIFFRACTION (2.2 Å)