3RGK

Crystal Structure of Human Myoglobin Mutant K45R

「2MM1」から置き換えられました

3RGK の概要

• エントリーDOI: 10.2210/pdb3rgk/pdb
• 分子名称: Myoglobin, PROTOPORPHYRIN IX CONTAINING FE, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: heme, oxygen transport
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17984.28

構造登録者

Hubbard, S.R. (登録日: 2011-04-08, 公開日: 2011-04-27, 最終更新日: 2023-09-13)

主引用文献

Hubbard, S.R.,Lambright, S.G.,Boxer, S.G.,Hendrickson, W.A.
X-ray crystal structure of a recombinant human myoglobin mutant at 2.8 A resolution
J.Mol.Biol., 20:215-218, 1990

PubMed Abstract: We have grown crystals in trigonal space group P3(2)21 of a mutant human myoglobin, aquomet form, in which lysine at position 45 has been replaced by arginine and cysteine at position 110 has been replaced by alanine. Suitable crystals of native recombinant human myoglobin have not been obtained. We have used the molecular replacement method to determine the X-ray crystal structure of the mutant at 2.8 A resolution. At the present stage of refinement, the crystallographic R-value for the model, with tightly restrained stereochemistry, is 0.158 for 5.0 to 2.8 A data. As expected, the overall structure is quite similar to the sperm whale myoglobin structure. Arginine 45 adopts a well-ordered conformation similar to that found in aquomet sperm whale myoglobin.

PubMed: 2342104

実験手法

X-RAY DIFFRACTION (1.65 Å)