3R85

Crystal structure of human SOUL BH3 domain in complex with Bcl-xL

3R85 の概要

• エントリーDOI: 10.2210/pdb3r85/pdb
• 関連するPDBエントリー: 3R8J 3R8K
• 分子名称: Bcl-2-like protein 1, Heme-binding protein 2, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: bcl-2-like protein, inhibitor of cell death, soul protein, apoptosis
• 由来する生物種: Homo sapiens; 詳細
• 細胞内の位置: Isoform Bcl-X(L): Mitochondrion inner membrane : Q07817; Cytoplasm : Q9Y5Z4
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 83112.45

構造登録者

Ambrosi, E.K.,Capaldi, S.,Bovi, M.,Saccomani, G.,Perduca, M.,Monaco, H.L. (登録日: 2011-03-23, 公開日: 2011-06-29, 最終更新日: 2023-09-13)

主引用文献

Ambrosi, E.,Capaldi, S.,Bovi, M.,Saccomani, G.,Perduca, M.,Monaco, H.L.
Structural changes in the BH3 domain of SOUL protein upon interaction with the anti-apoptotic protein Bcl-xL.
Biochem.J., 438:291-301, 2011

PubMed Abstract: The SOUL protein is known to induce apoptosis by provoking the mitochondrial permeability transition, and a sequence homologous with the BH3 (Bcl-2 homology 3) domains has recently been identified in the protein, thus making it a potential new member of the BH3-only protein family. In the present study, we provide NMR, SPR (surface plasmon resonance) and crystallographic evidence that a peptide spanning residues 147-172 in SOUL interacts with the anti-apoptotic protein Bcl-xL. We have crystallized SOUL alone and the complex of its BH3 domain peptide with Bcl-xL, and solved their three-dimensional structures. The SOUL monomer is a single domain organized as a distorted β-barrel with eight anti-parallel strands and two α-helices. The BH3 domain extends across 15 residues at the end of the second helix and eight amino acids in the chain following it. There are important structural differences in the BH3 domain in the intact SOUL molecule and the same sequence bound to Bcl-xL.

PubMed: 21639858
DOI: 10.1042/BJ20110257

実験手法

X-RAY DIFFRACTION (1.95 Å)