3R7F

Crystal Structure of CP-bound Aspartate Transcarbamoylase from Bacillus subtilis

3R7F の概要

• エントリーDOI: 10.2210/pdb3r7f/pdb
• 関連するPDBエントリー: 3R7D 3R7L
• 分子名称: Aspartate carbamoyltransferase, PHOSPHORIC ACID MONO(FORMAMIDE)ESTER, PHOSPHATE ION, ... (4 entities in total)
• 機能のキーワード: aspartate transcarbamoylase, carbamoyl phosphate, transferase, catalytic cycle
• 由来する生物種: Bacillus subtilis
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 104598.78

構造登録者

Cockrell, G.M.,Harris, K.M.,Puleo, D.E.,Kantrowitz, E.R. (登録日: 2011-03-22, 公開日: 2011-06-08, 最終更新日: 2024-02-21)

主引用文献

Harris, K.M.,Cockrell, G.M.,Puleo, D.E.,Kantrowitz, E.R.
Crystallographic Snapshots of the Complete Catalytic Cycle of the Unregulated Aspartate Transcarbamoylase from Bacillus subtilis.
J.Mol.Biol., 411:190-200, 2011

PubMed Abstract: Here, we report high-resolution X-ray structures of Bacillus subtilis aspartate transcarbamoylase (ATCase), an enzyme that catalyzes one of the first reactions in pyrimidine nucleotide biosynthesis. Structures of the enzyme have been determined in the absence of ligands, in the presence of the substrate carbamoyl phosphate, and in the presence of the bisubstrate/transition state analog N-phosphonacetyl-L-aspartate. Combining the structural data with in silico docking and electrostatic calculations, we have been able to visualize each step in the catalytic cycle of ATCase, from the ordered binding of the substrates, to the formation and decomposition of the tetrahedral intermediate, to the ordered release of the products from the active site. Analysis of the conformational changes associated with these steps provides a rationale for the lack of cooperativity in trimeric ATCases that do not possess regulatory subunits.

PubMed: 21663747
DOI: 10.1016/j.jmb.2011.05.036

実験手法

X-RAY DIFFRACTION (2.101 Å)