3R2X

Crystal structure of the de novo designed binding protein HB36.3 in complex the the 1918 influenza virus hemagglutinin

3R2X の概要

• エントリーDOI: 10.2210/pdb3r2x/pdb
• 分子名称: Hemagglutinin, HB36.3, designed hemagglutinin binding protein, alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: hemagglutinin, glycoprotein, viral protein-de novo protein complex, viral protein/de novo protein
• 由来する生物種: Influenza A virus; 詳細
• 細胞内の位置: Virion membrane; Single-pass type I membrane protein (Potential): Q9WFX3 Q9WFX3
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 68422.32

構造登録者

Ekiert, D.C.,Wilson, I.A. (登録日: 2011-03-14, 公開日: 2011-05-11, 最終更新日: 2024-11-27)

主引用文献

Fleishman, S.J.,Whitehead, T.A.,Ekiert, D.C.,Dreyfus, C.,Corn, J.E.,Strauch, E.M.,Wilson, I.A.,Baker, D.
Computational design of proteins targeting the conserved stem region of influenza hemagglutinin.
Science, 332:816-821, 2011

PubMed Abstract: We describe a general computational method for designing proteins that bind a surface patch of interest on a target macromolecule. Favorable interactions between disembodied amino acid residues and the target surface are identified and used to anchor de novo designed interfaces. The method was used to design proteins that bind a conserved surface patch on the stem of the influenza hemagglutinin (HA) from the 1918 H1N1 pandemic virus. After affinity maturation, two of the designed proteins, HB36 and HB80, bind H1 and H5 HAs with low nanomolar affinity. Further, HB80 inhibits the HA fusogenic conformational changes induced at low pH. The crystal structure of HB36 in complex with 1918/H1 HA revealed that the actual binding interface is nearly identical to that in the computational design model. Such designed binding proteins may be useful for both diagnostics and therapeutics.

PubMed: 21566186
DOI: 10.1126/science.1202617

実験手法

X-RAY DIFFRACTION (3.1 Å)