3QX9

Crystal structure of MID domain from hAGO2 in complex with ATP

3QX9 の概要

• エントリーDOI: 10.2210/pdb3qx9/pdb
• 関連するPDBエントリー: 3LUC 3QX8
• 分子名称: Protein argonaute-2, ADENOSINE-5'-TRIPHOSPHATE (3 entities in total)
• 機能のキーワード: rossmann-like fold, rna binding protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm, P-body: Q9UKV8
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 47499.41

構造登録者

Frank, F.,Fabian, M.R.,Stepinski, J.,Jemielity, J.,Darzynkiewicz, E.,Sonenberg, N.,Nagar, B. (登録日: 2011-03-01, 公開日: 2011-04-20, 最終更新日: 2024-02-21)

主引用文献

Frank, F.,Fabian, M.R.,Stepinski, J.,Jemielity, J.,Darzynkiewicz, E.,Sonenberg, N.,Nagar, B.
Structural analysis of 5'-mRNA-cap interactions with the human AGO2 MID domain.
Embo Rep., 12:415-420, 2011

PubMed Abstract: In RNA silencing, microRNA (miRNA)-mediated translational repression occurs through mechanisms that do not invoke messenger-RNA (mRNA) target cleavage by Argonaute proteins. The nature of these mechanisms is unclear, but several recent studies have proposed that a direct interaction between the mRNA-cap and the middle (MID) domain of Argonautes is involved. Here, we present crystallographic and NMR data demonstrating that cap analogues do not bind significantly to the isolated MID domain of human Argonaute 2 (hAGO2) and are found in the miRNA 5'-nucleotide binding site in an implausible binding mode. Additionally, in vitro pull-down experiments with full-length hAGO2 indicate that the interaction with cap analogues is nonspecific.

PubMed: 21475248
DOI: 10.1038/embor.2011.48

実験手法

X-RAY DIFFRACTION (2 Å)