3QUL

Crystal structures of the murine class I major histocompatibility complex H-2Db in complex with LCMV-derived gp33 altered peptide ligand (Y4S)

3QUL の概要

• エントリーDOI: 10.2210/pdb3qul/pdb
• 関連するPDBエントリー: 1S7U 1S7V 1S7W 1S7X 3QUK
• 分子名称: H-2 class I histocompatibility antigen, D-B alpha chain, Beta-2-microglobulin, Pre-glycoprotein polyprotein GP complex, ... (4 entities in total)
• 機能のキーワード: murine mhc, lcmv, receptor binding, beta2-microglobulin, immune system, t cell recognition, t cell receptor, cell surface
• 由来する生物種: Mus musculus (Mouse); 詳細
• 細胞内の位置: Membrane; Single-pass type I membrane protein: P01899; Secreted: P01887; Glycoprotein G1: Virion membrane ; Peripheral membrane protein . Glycoprotein G2: Virion membrane ; Single-pass membrane protein . Stable signal peptide: Virion membrane ; Multi-pass membrane protein : P07399
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 179044.79

構造登録者

Allerbring, E.,Duru, A.D.,Uchtenhagen, H.,Madhurantakam, C.,Grimm, S.,Tomek, M.B.,Mazumdar, P.A.,Spetz, A.,Friemann, R.,Sandalova, T.,Uhlin, M.,Nygren, P.,Achour, A. (登録日: 2011-02-24, 公開日: 2012-03-21, 最終更新日: 2023-09-13)

主引用文献

Allerbring, E.B.,Duru, A.D.,Uchtenhagen, H.,Madhurantakam, C.,Tomek, M.B.,Grimm, S.,Mazumdar, P.A.,Friemann, R.,Uhlin, M.,Sandalova, T.,Nygren, P.A.,Achour, A.
Unexpected T-cell recognition of an altered peptide ligand is driven by reversed thermodynamics.
Eur.J.Immunol., 42:2990-3000, 2012

PubMed Abstract: The molecular basis underlying T-cell recognition of MHC molecules presenting altered peptide ligands is still not well-established. A hierarchy of T-cell activation by MHC class I-restricted altered peptide ligands has been defined using the T-cell receptor P14 specific for H-2D(b) in complex with the immunodominant lymphocytic choriomeningitis virus peptide gp33 (KAVYNFATM). While substitution of tyrosine to phenylalanine (Y4F) or serine (Y4S) abolished recognition by P14, the TCR unexpectedly recognized H-2D(b) in complex with the alanine-substituted semiagonist Y4A, which displayed the most significant structural modification. The observed functional hierarchy gp33 > Y4A > Y4S = Y4F was neither due to higher stabilization capacity nor to differences in structural conformation. However, thermodynamic analysis demonstrated that while recognition of the full agonist H-2D(b) /gp33 was strictly enthalpy driven, recognition of the weak agonist H-2D(b) /Y4A was instead entropy driven with a large reduction in the favorable enthalpy term. The fourfold larger negative heat capacity derived for the interaction of P14 with H-2D(b) /gp33 compared with H-2D(b) /Y4A can possibly be explained by higher water entrapment at the TCR/MHC interface, which is also consistent with the measured opposite entropy contributions for the interactions of P14 with both MHCs. In conclusion, this study demonstrates that P14 makes use of different strategies to adapt to structural modifications in the MHC/peptide complex.

PubMed: 22837158
DOI: 10.1002/eji.201242588

実験手法

X-RAY DIFFRACTION (2 Å)