3QS6

Crystal structure of LeuT mutant F259V,I359Q bound to sodium and L-tryptophan

Summary for 3QS6

• Entry DOI: 10.2210/pdb3qs6/pdb
• Related: 2A65 3F3A 3QS4 3QS6
• Descriptor: Na(+):neurotransmitter symporter (Snf family), TRYPTOPHAN, SODIUM ION, ... (5 entities in total)
• Functional Keywords: nss, neurotransmitter transporter, leut-fold, sodium-coupled secondary transporter, sodium and amino acid binding, membrane, transport protein
• Biological source: Aquifex aeolicus
• Total number of polymer chains: 1
• Total formula weight: 59171.68

Authors

Piscitelli, C.L.,Gouaux, E. (deposition date: 2011-02-19, release date: 2011-10-12, Last modification date: 2023-09-13)

Primary citation

Piscitelli, C.L.,Gouaux, E.
Insights into transport mechanism from LeuT engineered to transport tryptophan.
Embo J., 31:228-235, 2012

PubMed Abstract: LeuT is a bacterial homologue of the neurotransmitter:sodium symporter (NSS) family and, being the only NSS member to have been structurally characterized by X-ray crystallography, is a model protein for studying transporter structure and mechanism. Transport activity in LeuT was hypothesized to require structural transitions between open-to-out and occluded conformations dependent upon protein:ligand binding complementarity. Here, using crystallographic and functional analysis, we show that binding site modification produces changes in both structure and activity that are consistent with complementarity-dependent structural transitions to the occluded state. The mutation I359Q converts the activity of tryptophan from inhibitor to transportable substrate. This mutation changes the local environment of the binding site, inducing the bound tryptophan to adopt a different conformer than in the wild-type complex. Instead of trapping the transporter open, tryptophan binding now allows the formation of an occluded state. Thus, transport activity is correlated to the ability of the ligand to promote the structural transition to the occluded state, a step in the transport cycle that is dependent on protein:ligand complementarity in the central binding site.

PubMed: 21952050
DOI: 10.1038/emboj.2011.353

Experimental method

X-RAY DIFFRACTION (2.801 Å)