3QO6

Crystal structure analysis of the plant protease Deg1

3QO6 の概要

• エントリーDOI: 10.2210/pdb3qo6/pdb
• 分子名称: Protease Do-like 1, chloroplastic, peptide, ... (6 entities in total)
• 機能のキーワード: protease, htra, ph-sensor, hydrolase, photosynthesis
• 由来する生物種: Arabidopsis thaliana (Thale-cress); 詳細
• 細胞内の位置: Plastid, chloroplast thylakoid membrane; Peripheral membrane protein; Lumenal side: O22609
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 112762.44

構造登録者

Clausen, T. (登録日: 2011-02-09, 公開日: 2011-05-04, 最終更新日: 2024-11-06)

主引用文献

Kley, J.,Schmidt, B.,Boyanov, B.,Stolt-Bergner, P.C.,Kirk, R.,Ehrmann, M.,Knopf, R.R.,Naveh, L.,Adam, Z.,Clausen, T.
Structural adaptation of the plant protease Deg1 to repair photosystem II during light exposure.
Nat.Struct.Mol.Biol., 18:728-731, 2011

PubMed Abstract: Deg1 is a chloroplastic protease involved in maintaining the photosynthetic machinery. Structural and biochemical analyses reveal that the inactive Deg1 monomer is transformed into the proteolytically active hexamer at acidic pH. The change in pH is sensed by His244, which upon protonation, repositions a specific helix to trigger oligomerization. This system ensures selective activation of Deg1 during daylight, when acidification of the thylakoid lumen occurs and photosynthetic proteins are damaged.

PubMed: 21532594
DOI: 10.1038/nsmb.2055

実験手法

X-RAY DIFFRACTION (2.5 Å)