3QO2

Structural insights for MPP8 chromodomain interaction with histone H3 lysine 9

3QO2 の概要

• エントリーDOI: 10.2210/pdb3qo2/pdb
• 分子名称: M-phase phosphoprotein 8, Histone H3 peptide, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: epigenetics, mpp8 phosphorylation, chromodomain, mpp8-h3k9me modulates the expression of e-cadherin, h3k9 methyl-lysine binding, tri-methyl-lysine, dna binding protein-gene regulation complex, histone h3 tail binding protein, dna binding protein/gene regulation
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus : Q99549
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 37072.49

構造登録者

Chang, Y.,Horton, J.R.,Bedford, M.T.,Zhang, X.,Cheng, X. (登録日: 2011-02-09, 公開日: 2011-04-06, 最終更新日: 2023-09-13)

主引用文献

Chang, Y.,Horton, J.R.,Bedford, M.T.,Zhang, X.,Cheng, X.
Structural insights for MPP8 chromodomain interaction with histone H3 lysine 9: potential effect of phosphorylation on methyl-lysine binding.
J.Mol.Biol., 408:807-814, 2011

PubMed Abstract: M-phase phosphoprotein 8 (MPP8) harbors an N-terminal chromodomain and a C-terminal ankyrin repeat domain. MPP8, via its chromodomain, binds histone H3 peptide tri- or di-methylated at lysine 9 (H3K9me3/H3K9me2) in submicromolar affinity. We determined the crystal structure of MPP8 chromodomain in complex with H3K9me3 peptide. MPP8 interacts with at least six histone H3 residues from glutamine 5 to serine 10, enabling its ability to distinguish lysine-9-containing peptide (QTARKS) from that of lysine 27 (KAARKS), both sharing the ARKS sequence. A partial hydrophobic cage with three aromatic residues (Phe59, Trp80 and Tyr83) and one aspartate (Asp87) encloses the methylated lysine 9. MPP8 has been reported to be phosphorylated in vivo, including the cage residue Tyr83 and the succeeding Thr84 and Ser85. Modeling a phosphate group onto the side-chain hydroxyl oxygen of Tyr83 suggests that the negatively charged phosphate group could enhance the binding of positively charged methyl-lysine or create a regulatory signal by allowing or inhibiting binding of other protein(s).

PubMed: 21419134
DOI: 10.1016/j.jmb.2011.03.018

実験手法

X-RAY DIFFRACTION (2.49 Å)