Summary for 3QM0
| Entry DOI | 10.2210/pdb3qm0/pdb |
| Descriptor | Histone acetyltransferase RTT109, MERCURY (II) ION, ACETYL COENZYME *A, ... (4 entities in total) |
| Functional Keywords | rtt109, histone acetyltransferase (hat), p300/cbp, histone h3 k56, genome stability, dna damage, dna repair, nucleus, transcription, transcription regulation, histone acetyltransferase (hat) fold, histone acetyltransferase, vps75 histone chaperone, auto-acetylation, lys290, transferase |
| Biological source | Saccharomyces cerevisiae (brewer's yeast,lager beer yeast,yeast) More |
| Cellular location | Nucleus : Q07794 |
| Total number of polymer chains | 1 |
| Total formula weight | 45860.10 |
| Authors | Tang, Y.,Marmorstein, R. (deposition date: 2011-02-03, release date: 2011-02-16, Last modification date: 2024-11-06) |
| Primary citation | Tang, Y.,Holbert, M.A.,Wurtele, H.,Meeth, K.,Rocha, W.,Gharib, M.,Jiang, E.,Thibault, P.,Verrault, A.,Cole, P.A.,Marmorstein, R. Fungal Rtt109 histone acetyltransferase is an unexpected structural homolog of metazoan p300/CBP. Nat.Struct.Mol.Biol., 15:738-745, 2008 Cited by PubMed Abstract: Rtt109, also known as KAT11, is a recently characterized fungal-specific histone acetyltransferase (HAT) that modifies histone H3 lysine 56 (H3K56) to promote genome stability. Rtt109 does not show sequence conservation with other known HATs and depends on association with either of two histone chaperones, Asf1 or Vps75, for HAT activity. Here we report the X-ray crystal structure of an Rtt109-acetyl coenzyme A complex and carry out structure-based mutagenesis, combined with in vitro biochemical studies of the Rtt109-Vps75 complex and studies of Rtt109 function in vivo. The Rtt109 structure reveals noteworthy homology to the metazoan p300/CBP HAT domain but exhibits functional divergence, including atypical catalytic properties and mode of cofactor regulation. The structure reveals a buried autoacetylated lysine residue that we show is also acetylated in the Rtt109 protein purified from yeast cells. Implications for understanding histone substrate and chaperone binding by Rtt109 are discussed. PubMed: 18568037DOI: 10.1038/nsmb.1448 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.1 Å) |
Structure validation
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