3QM0

Crystal structure of RTT109-AC-CoA complex

「3D35」から置き換えられました

3QM0 の概要

• エントリーDOI: 10.2210/pdb3qm0/pdb
• 分子名称: Histone acetyltransferase RTT109, MERCURY (II) ION, ACETYL COENZYME *A, ... (4 entities in total)
• 機能のキーワード: rtt109, histone acetyltransferase (hat), p300/cbp, histone h3 k56, genome stability, dna damage, dna repair, nucleus, transcription, transcription regulation, histone acetyltransferase (hat) fold, histone acetyltransferase, vps75 histone chaperone, auto-acetylation, lys290, transferase
• 由来する生物種: Saccharomyces cerevisiae (brewer's yeast,lager beer yeast,yeast); 詳細
• 細胞内の位置: Nucleus : Q07794
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 45860.10

構造登録者

Tang, Y.,Marmorstein, R. (登録日: 2011-02-03, 公開日: 2011-02-16, 最終更新日: 2024-11-06)

主引用文献

Tang, Y.,Holbert, M.A.,Wurtele, H.,Meeth, K.,Rocha, W.,Gharib, M.,Jiang, E.,Thibault, P.,Verrault, A.,Cole, P.A.,Marmorstein, R.
Fungal Rtt109 histone acetyltransferase is an unexpected structural homolog of metazoan p300/CBP.
Nat.Struct.Mol.Biol., 15:738-745, 2008

PubMed Abstract: Rtt109, also known as KAT11, is a recently characterized fungal-specific histone acetyltransferase (HAT) that modifies histone H3 lysine 56 (H3K56) to promote genome stability. Rtt109 does not show sequence conservation with other known HATs and depends on association with either of two histone chaperones, Asf1 or Vps75, for HAT activity. Here we report the X-ray crystal structure of an Rtt109-acetyl coenzyme A complex and carry out structure-based mutagenesis, combined with in vitro biochemical studies of the Rtt109-Vps75 complex and studies of Rtt109 function in vivo. The Rtt109 structure reveals noteworthy homology to the metazoan p300/CBP HAT domain but exhibits functional divergence, including atypical catalytic properties and mode of cofactor regulation. The structure reveals a buried autoacetylated lysine residue that we show is also acetylated in the Rtt109 protein purified from yeast cells. Implications for understanding histone substrate and chaperone binding by Rtt109 are discussed.

PubMed: 18568037
DOI: 10.1038/nsmb.1448

実験手法

X-RAY DIFFRACTION (3.1 Å)