3QIL

Crystal structure analysis of the clathrin trimerization domain

3QIL の概要

• エントリーDOI: 10.2210/pdb3qil/pdb
• 分子名称: Clathrin heavy chain 1 (1 entity in total)
• 機能のキーワード: clathrin trimerization domain, endocytosis, structural protein
• 由来する生物種: Bos taurus (bovine,cow,domestic cattle,domestic cow)
• 細胞内の位置: Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side: P49951
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 354210.65

構造登録者

Ybe, J.A.,Mishra, S.,Nix, J. (登録日: 2011-01-27, 公開日: 2012-02-01, 最終更新日: 2024-02-21)

主引用文献

Ybe, J.A.,Fontaine, S.N.,Stone, T.,Nix, J.,Lin, X.,Mishra, S.
Nuclear localization of clathrin involves a labile helix outside the trimerization domain.
Febs Lett., 587:142-149, 2013

PubMed Abstract: Clathrin is a trimeric protein involved in receptor-mediated-endocytosis, but can function as a non-trimer outside of endocytosis. We have discovered that the subcellular distribution of a clathrin cysteine mutant we previously studied is altered and a proportion is also localized to nuclear spaces. MALS shows C1573A hub is a mixture of trimer-like and detrimerized molecules. The X-ray structure of the trimerization domain reveals that without light chains, a helix harboring cysteine-1573 is reoriented. We propose clathrin has a detrimerization switch, which suggests clathrin topology can be altered naturally for new functions.

PubMed: 23178717
DOI: 10.1016/j.febslet.2012.11.005

実験手法

X-RAY DIFFRACTION (3.92 Å)