3Q98

Structure of ygeW encoded protein from E. coli

3Q98 の概要

• エントリーDOI: 10.2210/pdb3q98/pdb
• 分子名称: transcarbamylase (2 entities in total)
• 機能のキーワード: rossmann fold, unknown transcarbamylase, transferase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 45228.09

構造登録者

Li, Y.,Jing, Z.,Yu, X.,Allewell, N.M.,Tuchman, M.,Shi, D. (登録日: 2011-01-07, 公開日: 2011-05-04, 最終更新日: 2024-11-20)

主引用文献

Li, Y.,Jin, Z.,Yu, X.,Allewell, N.M.,Tuchman, M.,Shi, D.
The ygeW encoded protein from Escherichia coli is a knotted ancestral catabolic transcarbamylase.
Proteins, 79:2327-2334, 2011

PubMed Abstract: The allantoin degradation pathway in has long been thought to involve a putative novel oxamate transcarbamylase (OXTCase) that converts oxaluric acid to oxamate and carbamyl phosphate (CP), a substrate for carbamate kinase (CK). In the genome sequence of , the only gene that could encode a novel transcarbamylase is the gene. However, the recombinant protein has no transcarbamylase activity with oxamate, allantoin, or twenty five other related compounds as potential substrates. The crystal structures of this transcarbamylase with unknown function (UTCase) has been determined and refined at 2.0 Å resolution, providing structural insights into its possible function. Like -acetyl-L-ornithine transcarbamylase and -succinyl-L-transcarbamylase, UTCase has a deep 3 trefoil knot close to the active site, in contrast to aspartate transcarbamylase and ornithine transcarbamylase which do not have a knot. A Blast search of completed genomes indicates that 52 species including one non-bacterial species, G3, have the gene. Gene context analysis and the structure of UTCase suggest that it is probably an ancestral catabolic transcarbamylase.

PubMed: 21557323
DOI: 10.1002/prot.23043

実験手法

X-RAY DIFFRACTION (2.001 Å)