3Q7K

Formate Channel FocA from Salmonella typhimurium

3Q7K の概要

• エントリーDOI: 10.2210/pdb3q7k/pdb
• 分子名称: Probable formate transporter, FORMIC ACID (3 entities in total)
• 機能のキーワード: membrane protein, transport, cytoplasmic membrane, transport protein
• 由来する生物種: Salmonella enterica subsp. enterica serovar Typhi
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 321216.38

構造登録者

Lue, W.,Du, J.,Wacker, T.,Gerbig-Smentek, E.,Andrade, S.L.A.,Einsle, O. (登録日: 2011-01-05, 公開日: 2011-04-27, 最終更新日: 2023-11-01)

主引用文献

Lu, W.,Du, J.,Wacker, T.,Gerbig-Smentek, E.,Andrade, S.L.,Einsle, O.
pH-dependent gating in a FocA formate channel
Science, 332:352-354, 2011

PubMed Abstract: The formate transporter FocA was described to switch its mode of operation from a passive export channel at high external pH to a secondary active formate/H(+) importer at low pH. The crystal structure of Salmonella typhimurium FocA at pH 4.0 shows that this switch involves a major rearrangement of the amino termini of individual protomers in the pentameric channel. The amino-terminal helices open or block transport in a concerted, cooperative action that indicates how FocA is gated in a pH-dependent way. Electrophysiological studies show that the protein acts as a specific formate channel at pH 7.0 and that it closes upon a shift of pH to 5.1.

PubMed: 21493860
DOI: 10.1126/science.1199098

実験手法

X-RAY DIFFRACTION (2.8 Å)