3Q54

Crystal structure of Escherichia coli BamB

3Q54 の概要

• エントリーDOI: 10.2210/pdb3q54/pdb
• 分子名称: Outer membrane assembly lipoprotein YfgL (2 entities in total)
• 機能のキーワード: lipoprotein, bamb, lipid binding protein
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 39317.72

構造登録者

Dong, C.,Hou, H.,Yang, X. (登録日: 2010-12-27, 公開日: 2012-05-09, 最終更新日: 2024-02-21)

主引用文献

Dong, C.,Yang, X.,Hou, H.F.,Shen, Y.Q.,Dong, Y.H.
Structure of Escherichia coli BamB and its interaction with POTRA domains of BamA.
Acta Crystallogr.,Sect.D, 68:1134-1139, 2012

PubMed Abstract: In Escherichia coli, the BAM complex is essential for the assembly and insertion of outer membrane proteins (OMPs). The BAM complex is comprised of an integral β-barrel outer membrane protein BamA and four accessory lipoproteins BamB, BamC, BamD and BamE. Here, the crystal structure of BamB is reported. The crystal of BamB diffracted to 2.0 Å with one monomer in the asymmetric unit and the structure is composed of eight-bladed β-propeller motifs. Pull-down and Western blotting assays indicate that BamB interacts directly with the POTRA 1-3 domain of BamA and the C-terminal region of the POTRA 1-3 domain plays an important role in the interaction, while the POTRA 1-2 domain is not required for the interaction.

PubMed: 22948914
DOI: 10.1107/S0907444912023141

実験手法

X-RAY DIFFRACTION (2.004 Å)