3Q2T

Crystal Structure of CFIm68 RRM/CFIm25/RNA complex

Summary for 3Q2T

• Entry DOI: 10.2210/pdb3q2t/pdb
• Related: 3Q2S
• Descriptor: Cleavage and polyadenylation specificity factor subunit 5, Cleavage and polyadenylation specificity factor subunit 6, RNA, ... (4 entities in total)
• Functional Keywords: cfim, cfim25, cfim68, cpsf5, cpsf6, cpsf, 3' end processing, rna processing, cleavage factor, nudix protein, protein-protein complex, protein-rna complex, rrm, nudix fold, rna binding protein-rna complex, rna binding protein/rna
• Biological source: Homo sapiens (human); More
• Cellular location: Nucleus : O43809 Q16630
• Total number of polymer chains: 6
• Total formula weight: 100129.04

Authors

Yang, Q.,Coseno, M.,Gilmartin, G.M.,Doublie, S. (deposition date: 2010-12-20, release date: 2011-02-16, Last modification date: 2023-09-13)

Primary citation

Yang, Q.,Coseno, M.,Gilmartin, G.M.,Doublie, S.
Crystal Structure of a Human Cleavage Factor CFI(m)25/CFI(m)68/RNA Complex Provides an Insight into Poly(A) Site Recognition and RNA Looping.
Structure, 19:368-377, 2011

PubMed Abstract: Cleavage factor I(m) (CFI(m)) is a highly conserved component of the eukaryotic mRNA 3' processing machinery that functions in sequence-specific poly(A) site recognition through the collaboration of a 25 kDa subunit containing a Nudix domain and a larger subunit of 59, 68, or 72 kDa containing an RNA recognition motif (RRM). Our previous work demonstrated that CFI(m)25 is both necessary and sufficient for sequence-specific binding of the poly(A) site upstream element UGUA. Here, we report the crystal structure of CFI(m)25 complexed with the RRM domain of CFI(m)68 and RNA. The CFI(m)25 dimer is clasped on opposite sides by two CFI(m)68 RRM domains. Each CFI(m)25 subunit binds one UGUA element specifically. Biochemical analysis indicates that the CFI(m)68 RRMs serve to enhance RNA binding and facilitate RNA looping. The intrinsic ability of CFI(m) to direct RNA looping may provide a mechanism for its function in the regulation of alternative poly(A) site selection.

PubMed: 21295486
DOI: 10.1016/j.str.2010.12.021

Experimental method

X-RAY DIFFRACTION (3.061 Å)