3PVP

Structure of Mycobacterium tuberculosis DnaA-DBD in complex with box2 DNA

3PVP の概要

• エントリーDOI: 10.2210/pdb3pvp/pdb
• 関連するPDBエントリー: 3PVV
• 分子名称: Chromosomal replication initiator protein dnaA, DNA (5'-D(*CP*GP*TP*TP*AP*TP*CP*CP*AP*CP*AP*AP*C)-3'), DNA (5'-D(*GP*TP*TP*GP*TP*GP*GP*AP*TP*AP*AP*CP*G)-3'), ... (4 entities in total)
• 機能のキーワード: helical, dna replication, dna binding, dnaa-box, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Mycobacterium tuberculosis
• 細胞内の位置: Cytoplasm (By similarity): A5TY69
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 39033.15

構造登録者

Tsodikov, O.V.,Biswas, T. (登録日: 2010-12-07, 公開日: 2011-05-25, 最終更新日: 2024-02-21)

主引用文献

Tsodikov, O.V.,Biswas, T.
Structural and Thermodynamic Signatures of DNA Recognition by Mycobacterium tuberculosis DnaA.
J.Mol.Biol., 410:461-476, 2011

PubMed Abstract: An essential protein, DnaA, binds to 9-bp DNA sites within the origin of replication oriC. These binding events are prerequisite to forming an enigmatic nucleoprotein scaffold that initiates replication. The number, sequences, positions, and orientations of these short DNA sites, or DnaA boxes, within the oriCs of different bacteria vary considerably. To investigate features of DnaA boxes that are important for binding Mycobacterium tuberculosis DnaA (MtDnaA), we have determined the crystal structures of the DNA binding domain (DBD) of MtDnaA bound to a cognate MtDnaA-box (at 2.0 Å resolution) and to a consensus Escherichia coli DnaA-box (at 2.3 Å). These structures, complemented by calorimetric equilibrium binding studies of MtDnaA DBD in a series of DnaA-box variants, reveal the main determinants of DNA recognition and establish the [T/C][T/A][G/A]TCCACA sequence as a high-affinity MtDnaA-box. Bioinformatic and calorimetric analyses indicate that DnaA-box sequences in mycobacterial oriCs generally differ from the optimal binding sequence. This sequence variation occurs commonly at the first 2 bp, making an in vivo mycobacterial DnaA-box effectively a 7-mer and not a 9-mer. We demonstrate that the decrease in the affinity of these MtDnaA-box variants for MtDnaA DBD relative to that of the highest-affinity box TTGTCCACA is less than 10-fold. The understanding of DnaA-box recognition by MtDnaA and E. coli DnaA enables one to map DnaA-box sequences in the genomes of M. tuberculosis and other eubacteria.

PubMed: 21620858
DOI: 10.1016/j.jmb.2011.05.007

実験手法

X-RAY DIFFRACTION (2.3 Å)