3PVL

Structure of myosin VIIa MyTH4-FERM-SH3 in complex with the CEN1 of Sans

3PVL の概要

• エントリーDOI: 10.2210/pdb3pvl/pdb
• 分子名称: Myosin VIIa isoform 1, Usher syndrome type-1G protein, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: protein complex, novel folding, protein cargo binding, cargo proteins, motor protein-protein transport complex, motor protein/protein transport
• 由来する生物種: Mus musculus (mouse); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 85587.88

構造登録者

Wu, L.,Pan, L.F.,Wei, Z.Y.,Zhang, M.J. (登録日: 2010-12-07, 公開日: 2011-03-02, 最終更新日: 2024-03-20)

主引用文献

Wu, L.,Pan, L.,Wei, Z.,Zhang, M.
Structure of MyTH4-FERM domains in myosin VIIa tail bound to cargo.
Science, 331:757-760, 2011

PubMed Abstract: The unconventional myosin VIIa (MYO7A) is one of the five proteins that form a network of complexes involved in formation of stereocilia. Defects in these proteins cause syndromic deaf-blindness in humans [Usher syndrome I (USH1)]. Many disease-causing mutations occur in myosin tail homology 4-protein 4.1, ezrin, radixin, moesin (MyTH4-FERM) domains in the myosin tail that binds to another USH1 protein, Sans. We report the crystal structure of MYO7A MyTH4-FERM domains in complex with the central domain (CEN) of Sans at 2.8 angstrom resolution. The MyTH4 and FERM domains form an integral structural and functional supramodule binding to two highly conserved segments (CEN1 and 2) of Sans. The MyTH4-FERM/CEN complex structure provides mechanistic explanations for known deafness-causing mutations in MYO7A MyTH4-FERM. The structure will also facilitate mechanistic and functional studies of MyTH4-FERM domains in other myosins.

PubMed: 21311020
DOI: 10.1126/science.1198848

実験手法

X-RAY DIFFRACTION (2.8 Å)