3PTJ

Structural and functional Analysis of Arabidopsis thaliana thylakoid lumen protein AtTLP18.3

3PTJ の概要

• エントリーDOI: 10.2210/pdb3ptj/pdb
• 関連するPDBエントリー: 3PVH 3PW9
• 分子名称: UPF0603 protein At1g54780, chloroplastic (2 entities in total)
• 機能のキーワード: tap domain, rossmann fold, acid phosphatase, arabidopsis thaliana thylakoid lumen, hydrolase
• 由来する生物種: Arabidopsis thaliana (mouse-ear cress,thale-cress)
• 細胞内の位置: Plastid, chloroplast thylakoid membrane ; Single-pass membrane protein ; Lumenal side : Q9ZVL6
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16779.55

構造登録者

Wu, H.Y.,Liu, M.S.,Lin, T.P.,Cheng, Y.S. (登録日: 2010-12-03, 公開日: 2011-10-26, 最終更新日: 2024-10-16)

主引用文献

Wu, H.Y.,Liu, M.S.,Lin, T.P.,Cheng, Y.S.
Structural and functional assays of AtTLP18.3 identify its novel acid phosphatase activity in thylakoid lumen
Plant Physiol., 157:1015-1025, 2011

PubMed Abstract: The membrane protein AtTLP18.3 of Arabidopsis (Arabidopsis thaliana) contains a domain of unknown function, DUF477; it forms a polysome with photosynthetic apparatuses in the thylakoid lumen. To explore the molecular function of AtTLP18.3, we resolved its crystal structures with residues 83 to 260, the DUF477 only, and performed a series of biochemical analyses to discover its function. The gene expression of AtTLP18.3 followed a circadian rhythm. X-ray crystallography revealed the folding of AtTLP18.3 as a three-layer sandwich with three α-helices in the upper layer, four β-sheets in the middle layer, and two α-helices in the lower layer, which resembles a Rossmann fold. Structural comparison suggested that AtTLP18.3 might be a phosphatase. The enzymatic activity of AtTLP18.3 was further confirmed by phosphatase assay with various substrates (e.g. p-nitrophenyl phosphate, 6,8-difluoro-4-methylumbelliferyl phosphate, O-phospho-L-serine, and several synthetic phosphopeptides). Furthermore, we obtained the structure of AtTLP18.3 in complex with O-phospho-L-serine to identify the binding site of AtTLP18.3. Our structural and biochemical studies revealed that AtTLP18.3 has the molecular function of a novel acid phosphatase in the thylakoid lumen. DUF477 is accordingly renamed the thylakoid acid phosphatase domain.

PubMed: 21908686
DOI: 10.1104/pp.111.184739

実験手法

X-RAY DIFFRACTION (2.6 Å)