3PRC

PHOTOSYNTHETIC REACTION CENTER FROM RHODOPSEUDOMONAS VIRIDIS (QB-DEPLETED)

3PRC の概要

• エントリーDOI: 10.2210/pdb3prc/pdb
• 分子名称: PHOTOSYNTHETIC REACTION CENTER, SULFATE ION, MENAQUINONE-7, ... (13 entities in total)
• 機能のキーワード: photosynthetic reaction center, secondary quinone (qb)-depleted
• 由来する生物種: Blastochloris viridis; 詳細
• 細胞内の位置: Cell membrane; Lipid-anchor: P07173; Cellular chromatophore membrane; Multi-pass membrane protein (By similarity): P06009 P06010; Cellular chromatophore membrane; Single-pass membrane protein: P06008
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 143523.66

構造登録者

Lancaster, C.R.D.,Michel, H. (登録日: 1997-07-29, 公開日: 1998-11-11, 最終更新日: 2024-10-23)

主引用文献

Lancaster, C.R.,Michel, H.
The coupling of light-induced electron transfer and proton uptake as derived from crystal structures of reaction centres from Rhodopseudomonas viridis modified at the binding site of the secondary quinone, QB.
Structure, 5:1339-1359, 1997

PubMed Abstract: In a reaction of central importance to the energetics of photosynthetic bacteria, light-induced electron transfer in the reaction centre (RC) is coupled to the uptake of protons from the cytoplasm at the binding site of the secondary quinone (QB). In the original structure of the RC from Rhodopseudomonas viridis (PDB entry code 1PRC), the QB site was poorly defined because in the standard RC crystals it was only approximately 30% occupied with ubiquinone-9 (UQ9). We report here the structural characterization of the QB site by crystallographic refinement of UQ9-depleted RCs and of complexes of the RC either with ubiquinone-2 (UQ2) or the electron-transfer inhibitor stigmatellin in the QB site.

PubMed: 9351808
DOI: 10.1016/S0969-2126(97)00285-2

実験手法

X-RAY DIFFRACTION (2.4 Å)