3PR1

Crystal structure of apo Thermotoga maritima ribosome biogenesis GTP-binding protein EngB

3PR1 の概要

• エントリーDOI: 10.2210/pdb3pr1/pdb
• 関連するPDBエントリー: 3PQC
• 分子名称: Probable GTP-binding protein engB, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: rossmann fold, hydrolase, gtp binding, cell cycle
• 由来する生物種: Thermotoga maritima
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22582.39

構造登録者

Chan, K.H.,Wong, K.B. (登録日: 2010-11-29, 公開日: 2011-06-08, 最終更新日: 2023-11-01)

主引用文献

Chan, K.H.,Wong, K.B.
Structure of an essential GTPase, YsxC, from Thermotoga maritima
Acta Crystallogr.,Sect.F, 67:640-646, 2011

PubMed Abstract: YsxC belongs to the YihA family of TRAFAC class GTPases. The protein is involved in the biogenesis of ribosomes and is essential for the survival of a wide range of bacteria. Here, crystal structures of YsxC from Thermotoga maritima and its complex with GDP were determined at maximal resolutions of 2.3 and 1.9 Å, respectively. Major structural differences are observed in the switch I region, which is disordered in the apo form but exists in both an `open' and a `closed' conformation in the GDP-bound state. A comparison with the structure of the GMPPNP-YsxC complex from Bacillus subtilis provides insights into the mechanism of conformational change in the switch I and II regions upon hydrolysis of GTP.

PubMed: 21636901
DOI: 10.1107/S1744309111011651

実験手法

X-RAY DIFFRACTION (2.3 Å)