3PPH

Crystal structure of the Candida albicans methionine synthase by surface entropy reduction, threonine variant

3PPH の概要

• エントリーDOI: 10.2210/pdb3pph/pdb
• 関連するPDBエントリー: 3PPC 3PPF 3PPG
• 分子名称: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (2 entities in total)
• 機能のキーワード: cobalamin-independent, surface entropy reduction, zn binding, transferase
• 由来する生物種: Candida albicans (Yeast)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 176558.06

構造登録者

Ubhi, D.,Kavanagh, K.,Monzingo, A.F.,Robertus, J.D. (登録日: 2010-11-24, 公開日: 2011-10-12, 最終更新日: 2024-11-06)

主引用文献

Ubhi, D.,Kavanagh, K.L.,Monzingo, A.F.,Robertus, J.D.
Structure of Candida albicans methionine synthase determined by employing surface residue mutagenesis.
Arch.Biochem.Biophys., 513:19-26, 2011

PubMed Abstract: Fungal methionine synthase, Met6p, transfers a methyl group from 5-methyl-tetrahydrofolate to homocysteine to generate methionine. The enzyme is essential to fungal growth and is a potential anti-fungal drug design target. We have characterized the enzyme from the pathogen Candida albicans but were unable to crystallize it in native form. We converted Lys103, Lys104, and Glu107 all to Tyr (Met6pY), Thr (Met6pT) and Ala (Met6pA). All variants showed wild-type kinetic activity and formed useful crystals, each with unique crystal packing. In each case the mutated residues participated in beneficial crystal contacts. We have solved the three structures at 2.0-2.8Å resolution and analyzed crystal packing, active-site residues, and similarity to other known methionine synthase structures. C. albicans Met6p has a two domain structure with each of the domains having a (βα)(8)-barrel fold. The barrels are arranged face-to-face and the active site is located in a cleft between the two domains. Met6p utilizes a zinc ion for catalysis that is bound in the C-terminal domain and ligated by four conserved residues: His657, Cys659, Glu679 and Cys739.

PubMed: 21689631
DOI: 10.1016/j.abb.2011.06.002

実験手法

X-RAY DIFFRACTION (2.8 Å)