3PPE

Crystal structure of chicken VE-cadherin EC1-2

3PPE の概要

• エントリーDOI: 10.2210/pdb3ppe/pdb
• 分子名称: Vascular endothelial cadherin, CALCIUM ION (3 entities in total)
• 機能のキーワード: extracellular cadherin (ec) domain, beta barrel, ig-domain like, domain swapped dimer interface, calcium dependent cell-cell adhesion, cell surface, cell adhesion
• 由来する生物種: Gallus gallus (bantam,chickens)
• 細胞内の位置: Membrane; Single-pass type I membrane protein (By similarity): Q8AYD0
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 45627.20

構造登録者

Brasch, J.,Harrison, O.J.,Ahlsen, G.,Carnally, S.M.,Henderson, R.M.,Honig, B.,Shapiro, L.S. (登録日: 2010-11-24, 公開日: 2011-02-02, 最終更新日: 2023-09-06)

主引用文献

Brasch, J.,Harrison, O.J.,Ahlsen, G.,Carnally, S.M.,Henderson, R.M.,Honig, B.,Shapiro, L.
Structure and binding mechanism of vascular endothelial cadherin: a divergent classical cadherin.
J.Mol.Biol., 408:57-73, 2011

PubMed Abstract: Vascular endothelial cadherin (VE-cadherin), a divergent member of the type II classical cadherin family of cell adhesion proteins, mediates homophilic adhesion in the vascular endothelium. Previous investigations with a bacterially produced protein suggested that VE-cadherin forms cell surface trimers that bind between apposed cells to form hexamers. Here we report studies of mammalian-produced VE-cadherin ectodomains suggesting that, like other classical cadherins, VE-cadherin forms adhesive trans dimers between monomers located on opposing cell surfaces. Trimerization of the bacterially produced protein appears to be an artifact that arises from a lack of glycosylation. We also present the 2.1-Å-resolution crystal structure of the VE-cadherin EC1-2 adhesive region, which reveals homodimerization via the strand-swap mechanism common to classical cadherins. In common with type II cadherins, strand-swap binding involves two tryptophan anchor residues, but the adhesive interface resembles type I cadherins in that VE-cadherin does not form a large nonswapped hydrophobic surface. Thus, VE-cadherin is an outlier among classical cadherins, with characteristics of both type I and type II subfamilies.

PubMed: 21269602
DOI: 10.1016/j.jmb.2011.01.031

実験手法

X-RAY DIFFRACTION (2.1 Å)