3POE

Crystal structure of the MASP-1 CUB2 domain bound to Ca2+

3POE の概要

• エントリーDOI: 10.2210/pdb3poe/pdb
• 関連するPDBエントリー: 3POB
• 分子名称: Mannan-binding lectin serine protease 1, CALCIUM ION (3 entities in total)
• 機能のキーワード: cub domain, ca2+ binding site, complement protein, lectin pathway of complement, mbl, mbp, bloodstream, hydrolase
• 由来する生物種: Rattus norvegicus (brown rat,rat,rats)
• 細胞内の位置: Secreted: Q8CHN8
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13171.67

構造登録者

Gingras, A.R.,Moody, P.C.E.,Wallis, R. (登録日: 2010-11-22, 公開日: 2011-08-24, 最終更新日: 2024-10-30)

主引用文献

Gingras, A.R.,Girija, U.V.,Keeble, A.H.,Panchal, R.,Mitchell, D.A.,Moody, P.C.,Wallis, R.
Structural Basis of Mannan-Binding Lectin Recognition by Its Associated Serine Protease MASP-1: Implications for Complement Activation.
Structure, 19:1635-1643, 2011

PubMed Abstract: Complement activation contributes directly to health and disease. It neutralizes pathogens and stimulates immune processes. Defects lead to immunodeficiency and autoimmune diseases, whereas inappropriate activation causes self-damage. In the lectin and classical pathways, complement is triggered upon recognition of a pathogen by an activating complex. Here we present the first structure of such a complex in the form of the collagen-like domain of mannan-binding lectin (MBL) and the binding domain of its associated protease (MASP-1/-3). The collagen binds within a groove using a pivotal lysine side chain that interacts with Ca(2+)-coordinating residues, revealing the essential role of Ca(2+). This mode of binding is prototypic for all activating complexes of the lectin and classical pathways, and suggests a general mechanism for the global changes that drive activation. The structural insights reveal a new focus for inhibitors and we have validated this concept by targeting the binding pocket of the MASP.

PubMed: 22078562
DOI: 10.1016/j.str.2011.08.014

実験手法

X-RAY DIFFRACTION (1.501 Å)