3PO3

Arrested RNA Polymerase II reactivation intermediate

3PO3 の概要

• エントリーDOI: 10.2210/pdb3po3/pdb
• 関連するPDBエントリー: 3PO2
• 分子名称: DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerase II subunit RPB11, ... (21 entities in total)
• 機能のキーワード: rna polymerase ii, mrna, transcription, arrest, backtracking, cleavage, transferase-dna-rna complex, transferase/dna/rna
• 由来する生物種: Saccharomyces cerevisiae (brewer's yeast,lager beer yeast,yeast); 詳細
• 細胞内の位置: Nucleus: P04050 P38902 P07273 P08518 P16370 P20433 P20434 P34087 P20436; Nucleus, nucleolus : P22139 P40422 P27999; Cytoplasm : P20435
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 549499.53

構造登録者

Cheung, A.C.M.,Cramer, P. (登録日: 2010-11-21, 公開日: 2011-03-02, 最終更新日: 2023-09-06)

主引用文献

Cheung, A.C.,Cramer, P.
Structural basis of RNA polymerase II backtracking, arrest and reactivation.
Nature, 471:249-253, 2011

PubMed Abstract: During gene transcription, RNA polymerase (Pol) II moves forwards along DNA and synthesizes messenger RNA. However, at certain DNA sequences, Pol II moves backwards, and such backtracking can arrest transcription. Arrested Pol II is reactivated by transcription factor IIS (TFIIS), which induces RNA cleavage that is required for cell viability. Pol II arrest and reactivation are involved in transcription through nucleosomes and in promoter-proximal gene regulation. Here we present X-ray structures at 3.3 Å resolution of an arrested Saccharomyces cerevisiae Pol II complex with DNA and RNA, and of a reactivation intermediate that additionally contains TFIIS. In the arrested complex, eight nucleotides of backtracked RNA bind a conserved 'backtrack site' in the Pol II pore and funnel, trapping the active centre trigger loop and inhibiting mRNA elongation. In the reactivation intermediate, TFIIS locks the trigger loop away from backtracked RNA, displaces RNA from the backtrack site, and complements the polymerase active site with a basic and two acidic residues that may catalyse proton transfers during RNA cleavage. The active site is demarcated from the backtrack site by a 'gating tyrosine' residue that probably delimits backtracking. These results establish the structural basis of Pol II backtracking, arrest and reactivation, and provide a framework for analysing gene regulation during transcription elongation.

PubMed: 21346759
DOI: 10.1038/nature09785

実験手法

X-RAY DIFFRACTION (3.3 Å)