3PN7

Visualizing new hinges and a potential major source of compliance in the lever arm of myosin

3PN7 の概要

• エントリーDOI: 10.2210/pdb3pn7/pdb
• 関連するPDBエントリー: 1WDC 3JTD 3JVT
• 分子名称: Myosin heavy chain, Myosin regulatory light chain, Myosin essential light chain, ... (6 entities in total)
• 機能のキーワード: alpha helix, muscle contraction, calcium binding, catch muscle, protein binding
• 由来する生物種: Placopecten magellanicus (Sea scallop); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 89492.21

構造登録者

Brown, J.H.,Senthil-Kumar, V.S.,O'Neall-Hennessey, E.,Reshetnikova, L.,Robinson, H.,Nguyen-McCarty, M.,Szent-Gyorgyi, A.G.,Cohen, C. (登録日: 2010-11-18, 公開日: 2011-01-05, 最終更新日: 2023-09-06)

主引用文献

Brown, J.H.,Senthil Kumar, V.S.,O'Neall-Hennessey, E.,Reshetnikova, L.,Robinson, H.,Nguyen-McCarty, M.,Szent-Gyorgyi, A.G.,Cohen, C.
Visualizing key hinges and a potential major source of compliance in the lever arm of myosin.
Proc.Natl.Acad.Sci.USA, 108:114-119, 2011

PubMed Abstract: We have determined the 2.3-Å-resolution crystal structure of a myosin light chain domain, corresponding to one type found in sea scallop catch ("smooth") muscle. This structure reveals hinges that may function in the "on" and "off" states of myosin. The molecule adopts two different conformations about the heavy chain "hook" and regulatory light chain (RLC) helix D. This conformational change results in extended and compressed forms of the lever arm whose lengths differ by 10 Å. The heavy chain hook and RLC helix D hinges could thus serve as a potential major and localized source of cross-bridge compliance during the contractile cycle. In addition, in one of the molecules of the crystal, part of the RLC N-terminal extension is seen in atomic detail and forms a one-turn alpha-helix that interacts with RLC helix D. This extension, whose sequence is highly variable in different myosins, may thus modulate the flexibility of the lever arm. Moreover, the relative proximity of the phosphorylation site to the helix D hinge suggests a potential role for conformational changes about this hinge in the transition between the on and off states of regulated myosins.

PubMed: 21149681
DOI: 10.1073/pnas.1016288107

実験手法

X-RAY DIFFRACTION (2.25 Å)